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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 501/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction enzyme के repeated warming से क्या असर हो सकता है?

What can be the effect of repeated warming of a restriction enzyme?

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A. enzyme stability घट सकती हैEnzyme stability can decrease

Explanation

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Restriction enzymes proteins हैं और heat sensitive हो सकते हैं। उन्हें ice पर रखें और जल्दी freezer में वापस रखें। / Restriction enzymes are proteins and can be heat sensitive. Keep them on ice and return quickly to freezer.

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Question 502/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में enzyme को pipette करते समय tip को stock में बार-बार क्यों नहीं डालना चाहिए?

Why should the tip not be inserted repeatedly into enzyme stock during restriction digestion?

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C. contamination risk घटाने के लिएTo reduce contamination risk

Explanation

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Repeated entry contamination और enzyme loss का risk बढ़ाती है। Fresh sterile tip और careful pipetting use करें। / Repeated entry increases risk of contamination and enzyme loss. Use fresh sterile tip and careful pipetting.

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Question 503/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में buffer thaw होने के बाद precipitate दिखे तो क्या करना चाहिए?

What should be done if precipitate appears in buffer after thawing in restriction digestion?

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D. manufacturer instruction के अनुसार mix या warm करेंMix or warm according to manufacturer instruction

Explanation

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Buffer precipitate salt imbalance दिखा सकता है। Correct buffer condition enzyme activity के लिए जरूरी है। / Buffer precipitate can indicate salt imbalance. Correct buffer condition is needed for enzyme activity.

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Question 504/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में buffer को well mixed करना क्यों जरूरी है?

Why is it necessary to mix buffer well in restriction digestion?

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A. salt और cofactors समान concentration में रहेंTo keep salt and cofactors at uniform concentration

Explanation

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Buffer components uneven हों तो enzyme activity प्रभावित हो सकती है। Gentle mixing reaction reliability बढ़ाता है। / If buffer components are uneven enzyme activity can be affected. Gentle mixing increases reaction reliability.

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Question 505/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion reaction में final buffer concentration आमतौर पर कैसी रखी जाती है?

How is final buffer concentration usually kept in restriction digestion reaction?

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B. manufacturer recommended working concentration

Explanation

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Recommended working buffer concentration enzyme activity के लिए optimized होती है। बहुत dilute या concentrated buffer result खराब कर सकता है। / Recommended working buffer concentration is optimized for enzyme activity. Too dilute or too concentrated buffer can spoil result.

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Question 506/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction enzyme के साथ incompatible buffer use करने से क्या हो सकता है?

What can happen by using incompatible buffer with restriction enzyme?

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C. enzyme activity घट सकती हैEnzyme activity can decrease

Explanation

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गलत buffer pH salt या cofactor mismatch दे सकता है। इससे partial digestion या no digestion हो सकता है। / Wrong buffer can cause pH salt or cofactor mismatch. This may lead to partial or no digestion.

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Question 507/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में two-enzyme compatibility table क्यों देखी जाती है?

Why is a two-enzyme compatibility table checked in restriction digestion?

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A. दोनों enzymes एक buffer में कितने active हैं जानने के लिएTo know how active both enzymes are in one buffer

Explanation

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Double digest में दोनों enzymes को suitable condition चाहिए। Compatibility table one-pot या sequential plan तय करती है। / Both enzymes need suitable conditions in double digest. Compatibility table decides one-pot or sequential plan.

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Question 508/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

अगर double digest के enzymes incompatible buffers मांगते हैं तो कौन सा तरीका उपयोगी है?

If enzymes in double digest need incompatible buffers which method is useful?

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A. sequential digestion

Explanation

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Sequential digestion में enzymes अलग-अलग conditions में use होते हैं। इससे दोनों enzymes को उचित environment मिलता है। / In sequential digestion enzymes are used under separate conditions. This gives both enzymes suitable environment.

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Question 509/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Sequential digestion में पहले enzyme के बाद cleanup कब जरूरी हो सकता है?

When can cleanup after the first enzyme be necessary in sequential digestion?

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A. जब दूसरा buffer पहले से incompatible होWhen second buffer is incompatible with first

Explanation

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पहले reaction के salts या enzyme दूसरे reaction को affect कर सकते हैं। Cleanup या buffer exchange helpful हो सकता है। / Salts or enzyme from first reaction can affect the second reaction. Cleanup or buffer exchange can help.

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Question 510/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Double digest में यदि एक enzyme heat-inactivated नहीं होता तो क्या किया जा सकता है?

What can be done if one enzyme in double digest is not heat-inactivated?

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A. cleanup या enzyme removal step use करेंUse cleanup or enzyme removal step

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Non-inactivated enzyme next step में activity रख सकता है। Cleanup unwanted cutting रोकने में मदद करता है। / A non-inactivated enzyme can remain active in next step. Cleanup helps stop unwanted cutting.

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