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11 results found for "carryover" in all classes.

Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में enzyme carryover ligation में क्यों risk है?

Why is enzyme carryover risky in ligation after restriction digestion?

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A. ligated DNA को फिर cut कर सकता हैIt can cut ligated DNA again

Explanation

Simple Explanation

Active restriction enzyme newly ligated DNA को काट सकता है। इसलिए inactivation या purification use करें। / Active restriction enzyme can cut newly ligated DNA. Therefore use inactivation or purification.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest purification में ethanol carryover क्यों avoid करना चाहिए?

Why should ethanol carryover be avoided in restriction digest purification?

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A. ligation और enzyme reactions inhibit हो सकती हैंLigation and enzyme reactions can be inhibited

Explanation

Simple Explanation

Residual ethanol downstream reactions को रोक सकता है। Purified DNA को सही तरह dry करें। / Residual ethanol can inhibit downstream reactions. Dry purified DNA properly.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में ethanol carryover कैसे पता चल सकता है?

How can ethanol carryover be suspected in restriction digest?

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A. poor enzyme reaction और wet DNA pellet history सेFrom poor enzyme reaction and wet DNA pellet history

Explanation

Simple Explanation

Residual ethanol enzyme reaction inhibit कर सकता है। Pellet proper dry होना चाहिए। / Residual ethanol can inhibit enzyme reaction. Pellet should be properly dried.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में phenol carryover का क्या असर हो सकता है?

What can be the effect of phenol carryover in restriction digest?

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Correct Answer

A. enzyme inhibition

Explanation

Simple Explanation

Phenol enzyme proteins को affect कर सकता है। Clean DNA digestion के लिए जरूरी है। / Phenol can affect enzyme proteins. Clean DNA is necessary for digestion.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 51

Restriction digest में sample carryover contamination कैसे रोकी जा सकती है?

How can sample carryover contamination be prevented in restriction digest?

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A. fresh tips और careful pipetting सेWith fresh tips and careful pipetting

Explanation

Simple Explanation

Fresh tips sample mixing को रोकती हैं। Careful pipetting reliable result देती है। / Fresh tips prevent sample mixing. Careful pipetting gives reliable results.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 42

DNA sample में salt carryover क्यों समस्या है?

Why is salt carryover a problem in a DNA sample?

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Correct Answer

C. downstream enzyme reactions रोक सकता हैIt can inhibit downstream enzyme reactions

Explanation

Simple Explanation

Excess salt PCR और enzyme digestion को inhibit कर सकता है। Wash step salt removal में मदद करता है। / Excess salt can inhibit PCR and enzyme digestion. Wash step helps remove salt.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 41

DNA isolation में ethanol carryover कैसे घटाया जा सकता है?

How can ethanol carryover be reduced in DNA isolation?

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A. pellet या column को ठीक से dry करकेBy properly drying pellet or column

Explanation

Simple Explanation

Proper drying residual ethanol हटाने में मदद करती है। Ethanol carryover downstream reactions रोक सकता है। / Proper drying helps remove residual ethanol. Ethanol carryover can inhibit downstream reactions.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 41

DNA sample में salt carryover से क्या effect हो सकता है?

What effect can salt carryover have in a DNA sample?

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Correct Answer

A. downstream enzyme reactions प्रभावित हो सकती हैंDownstream enzyme reactions can be affected

Explanation

Simple Explanation

Excess salt PCR या enzyme digestion को inhibit कर सकता है। Wash step salt removal में मदद करता है। / Excess salt can inhibit PCR or enzyme digestion. Wash step helps salt removal.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product में salts बचने से ligation पर क्या असर हो सकता है?

What effect can remaining salts in restriction digestion product have on ligation?

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Correct Answer

A. ligation efficiency घट सकती हैLigation efficiency can decrease

Explanation

Simple Explanation

Excess salts ligase और DNA end interaction को affect कर सकते हैं। Purification और wash step जरूरी हैं। / Excess salts can affect ligase and DNA end interaction. Purification and wash step are necessary.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में purified fragment में salt बचा हो तो ligation पर क्या असर होगा?

What effect can leftover salt in purified fragment have on ligation?

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Correct Answer

A. ligation efficiency घट सकती हैLigation efficiency can decrease

Explanation

Simple Explanation

Salt carryover ligase reaction को प्रभावित कर सकता है। Final wash और drying सही करें। / Salt carryover can affect ligase reaction. Do final wash and drying properly.

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Question Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme reaction के लिए DNA purity का कौन सा issue सबसे problematic हो सकता है?

Which DNA purity issue can be very problematic for restriction enzyme reaction?

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Correct Answer

A. phenol ethanol या salt carryoverPhenol ethanol or salt carryover

Explanation

Simple Explanation

Phenol ethanol और salts enzyme activity inhibit कर सकते हैं। Digestion से पहले cleanup useful हो सकता है। / Phenol ethanol and salts can inhibit enzyme activity. Cleanup can be useful before digestion.

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