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Biotechnology Restriction Enzymes MCQ Questions for Class 11 Science

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01 Practice Topic Genetic Engineering 200 MCQs 02 Practice Topic Bioprocess Engineering 300 MCQs 03 Practice Topic Ligase Enzymes 290 MCQs 04 Practice Topic Cloning Vectors 295 MCQs 05 Practice Topic Competent Host 445 MCQs

Restriction Enzymes Practice Questions

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Question 171/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

RFLP में restriction enzyme क्यों जरूरी होता है?

Why is a restriction enzyme needed in RFLP?

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A. DNA को specific fragments में काटने के लिएTo cut DNA into specific fragments

Explanation

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RFLP में fragment length differences restriction cuts के बाद देखे जाते हैं। इसलिए restriction enzyme पहला महत्वपूर्ण उपकरण है। / In RFLP fragment length differences are observed after restriction cuts. Therefore restriction enzyme is the first important tool.

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Question 172/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक forensic sample में DNA fragments की तुलना की जा रही है। restriction enzymes का role क्या है?

DNA fragments are being compared in a forensic sample. What is the role of restriction enzymes?

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A. DNA को pattern बनाने वाले fragments में काटनाCutting DNA into fragments that form a pattern

Explanation

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Restriction enzymes DNA को repeatable pattern वाले fragments में काट सकते हैं। यह DNA comparison में मदद करता है। / Restriction enzymes can cut DNA into fragments with a repeatable pattern. This helps in DNA comparison.

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Question 173/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

कौन सा test question restriction enzyme specificity को सबसे अच्छा दिखाता है?

Which test question best shows restriction enzyme specificity?

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A. कौन सा enzyme किस sequence को पहचानता हैWhich enzyme recognizes which sequence

Explanation

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Specificity sequence recognition से जुड़ी है। इसलिए enzyme-sequence matching इसका सही test है। / Specificity is linked with sequence recognition. Therefore enzyme-sequence matching tests it correctly.

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Question 174/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

अगर दो DNA fragments के sticky ends complementary नहीं हैं तो क्या समस्या होगी?

If sticky ends of two DNA fragments are not complementary what problem will occur?

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A. वे आसानी से pair नहीं करेंगेThey will not pair easily

Explanation

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Sticky ends को जुड़ने के लिए complementarity चाहिए। बिना complementarity ligation कठिन होगी। / Sticky ends need complementarity to join. Without complementarity ligation will be difficult.

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Question 175/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक cloning experiment में insert और vector के ends compatible हैं। अगला मुख्य enzyme कौन सा होगा?

In a cloning experiment insert and vector ends are compatible. Which enzyme is mainly needed next?

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Correct Answer

A. DNA ligase

Explanation

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Compatible ends को स्थायी रूप से जोड़ने के लिए DNA ligase चाहिए। Restriction enzymes पहले cutting में काम करते हैं। / DNA ligase is needed to permanently join compatible ends. Restriction enzymes act earlier in cutting.

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Question 176/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किस विकल्प में restriction enzyme और product का सही जोड़ा है?

Which option correctly pairs restriction enzyme with product?

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A. EcoRI-sticky ends

Explanation

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EcoRI जैसे enzymes DNA cuts और sticky ends दे सकते हैं। ये chlorophyll या glucose नहीं बनाते। / Enzymes like EcoRI make DNA cuts and can produce sticky ends. They do not make chlorophyll or glucose.

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Question 177/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

कौन सा practical safety idea restriction enzymes के साथ सही है?

Which practical safety idea is correct with restriction enzymes?

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A. ठंडी storage और सही buffer का उपयोगUse cold storage and proper buffer

Explanation

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Restriction enzymes proteins हैं और conditions sensitive हो सकते हैं। सही storage और buffer activity बचाते हैं। / Restriction enzymes are proteins and can be condition-sensitive. Proper storage and buffer preserve activity.

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Question 178/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

यदि एक enzyme बहुत अधिक मात्रा में गलत buffer में डाला गया तो किस effect का खतरा बढ़ सकता है?

If an enzyme is added in excess in a wrong buffer what effect may increase?

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A. star activity

Explanation

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Excess enzyme और गलत buffer non-specific cutting बढ़ा सकते हैं। इसे star activity कहते हैं। / Excess enzyme and wrong buffer can increase non-specific cutting. This is called star activity.

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Question 179/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किसी DNA में methylated recognition site है। expected cut क्यों नहीं मिला?

A DNA has a methylated recognition site. Why was the expected cut not obtained?

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Correct Answer

A. methylation ने enzyme cutting रोक दी हो सकती हैMethylation may have blocked enzyme cutting

Explanation

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कुछ enzymes methylated sites को काट नहीं पाते। इसलिए expected restriction cut नहीं दिख सकता। / Some enzymes cannot cut methylated sites. Therefore the expected restriction cut may not appear.

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Question 180/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक student ने कहा कि all restriction enzymes same sequence पहचानते हैं। सही प्रतिक्रिया क्या है?

A student says all restriction enzymes recognize the same sequence. What is the correct response?

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Correct Answer

A. गलत क्योंकि अलग enzymes अलग sequences पहचान सकते हैंIncorrect because different enzymes can recognize different sequences

Explanation

Simple Explanation

Restriction enzymes sequence-specific होते हैं और sequence enzyme के अनुसार बदल सकता है। यह fundamental fact है। / Restriction enzymes are sequence-specific and the sequence can differ by enzyme. This is a fundamental fact.

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