Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 151-160 of 345 questions.
A. तीनThree
वृत्ताकार डीएनए में पूर्ण पाचन पर खंडों की संख्या कटों के बराबर होती है। तीन कट से तीन खंड मिलेंगे। / In circular DNA after complete digestion fragments equal the number of cuts. Three cuts give three fragments.
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If linear DNA has no BamHI site what will happen after adding BamHI?
A. डीएनए नहीं कटेगाDNA will not be cut
एंजाइम को कट करने के लिए अपना पहचान स्थल चाहिए। BamHI स्थल न हो तो BamHI कट नहीं करेगा। / The enzyme needs its recognition site to cut. If there is no BamHI site BamHI will not cut.
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After restriction digestion several bands appear in a gel. What do these bands represent?
A. अलग आकार के डीएनए खंडDNA fragments of different sizes
जेल बैंड अलग-अलग लंबाई के डीएनए खंड दिखाते हैं। परीक्षा में बैंड को fragment size से जोड़ें। / Gel bands show DNA fragments of different lengths. In exams link bands with fragment size.
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In which condition is the chance of a desired gene inserting in reverse orientation reduced?
A. दो अलग प्रतिबंध एंजाइमों से directional cloning करनाDoing directional cloning with two different restriction enzymes
दो अलग सिरे insert की दिशा नियंत्रित करने में मदद करते हैं। इसे directional cloning का लाभ मानें। / Two different ends help control insert orientation. Treat this as an advantage of directional cloning.
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If ligase is not added what problem will occur with cut vector and insert?
A. स्थायी जोड़ बनना कठिन होगाPermanent joining will be difficult
Restriction enzyme सिरों को बनाता है पर ligase स्थायी बंध बनाता है। इसलिए ligase जरूरी है। / Restriction enzyme makes the ends but ligase forms permanent bonds. Therefore ligase is needed.
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A student says sticky ends join with complementary ends. Which principle applies?
A. आधार युग्मनBase pairing
चिपचिपे सिरों में पूरक आधार अस्थायी युग्मन कर सकते हैं। यही ligation को आसान बनाता है। / Complementary bases in sticky ends can temporarily pair. This makes ligation easier.
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Which MCQ option gives the correct recognition sequence of EcoRI?
A. GAATTC
EcoRI GAATTC sequence को पहचानता है। इसे common restriction enzyme example के रूप में याद रखें। / EcoRI recognizes the GAATTC sequence. Remember it as a common restriction enzyme example.
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Which option has the correct recognition sequence of BamHI?
A. GGATCC
BamHI GGATCC क्रम पहचानता है। enzyme-sequence matching में यह महत्वपूर्ण उदाहरण है। / BamHI recognizes the GGATCC sequence. It is an important example in enzyme-sequence matching.
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Which option gives the correct recognition sequence of HindIII?
A. AAGCTT
HindIII AAGCTT पहचान क्रम से जुड़ा है। इसे EcoRI और BamHI से अलग याद रखें। / HindIII is associated with the AAGCTT recognition sequence. Remember it separately from EcoRI and BamHI.
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Which option correctly matches HaeIII with its ends?
A. HaeIII-मंद सिरेHaeIII-blunt ends
HaeIII को blunt end example के रूप में पढ़ा जाता है। यह GGCC क्रम से भी जुड़ा है। / HaeIII is studied as a blunt end example. It is also linked with the GGCC sequence.
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