Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 161-170 of 345 questions.
Why is a DNA ladder loaded in a class experiment?
A. खंडों के आकार का अनुमान लगाने के लिएTo estimate fragment sizes
DNA ladder आकार का मानक देता है। इससे sample bands की length का अनुमान लगाया जाता है। / A DNA ladder gives a size standard. It helps estimate the length of sample bands.
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If the expected band is not seen after restriction digestion what can be one simple reason?
A. अपूर्ण पाचन या कम डीएनएIncomplete digestion or low DNA
अपूर्ण पाचन या कम DNA से band कमजोर या absent दिख सकता है। practical questions में ऐसे कारण याद रखें। / Incomplete digestion or low DNA can make a band weak or absent. Remember such reasons for practical questions.
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An extra larger band appears in a sample. What may it indicate?
A. अपूर्ण पाचनIncomplete digestion
अपूर्ण पाचन में कुछ DNA molecules पूरी तरह नहीं कटते। इसलिए बड़े bands दिख सकते हैं। / In incomplete digestion some DNA molecules are not fully cut. Therefore larger bands may appear.
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A bacterium methylates its DNA. What is the benefit against restriction enzymes?
A. अपना DNA कटने से बचता हैIts own DNA is protected from cutting
मेथिलेशन self DNA को पहचानकर कटने से बचा सकता है। यह restriction-modification system का हिस्सा है। / Methylation can protect self DNA from being cut. This is part of the restriction-modification system.
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Phage DNA enters a bacterium. What can be the possible role of a restriction enzyme?
A. फेज DNA को काटनाCutting phage DNA
Restriction enzymes foreign phage DNA को काटकर bacterial defense में मदद करते हैं। यह natural role है। / Restriction enzymes help bacterial defense by cutting foreign phage DNA. This is their natural role.
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In which condition may star activity be suspected?
A. एंजाइम गलत conditions में non-specific cut करेEnzyme cuts non-specifically under wrong conditions
Star activity में enzyme अपने normal recognition से बाहर भी काट सकता है। यह improper reaction conditions से जुड़ा हो सकता है। / In star activity an enzyme may cut outside its normal recognition specificity. It can be linked with improper reaction conditions.
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The buffer was wrong in an experiment. What may happen to restriction enzyme activity?
A. activity घट सकती है या बदल सकती हैActivity may decrease or change
बफर enzyme के लिए सही pH और ions देता है। गलत buffer कटिंग को प्रभावित कर सकता है। / Buffer provides proper pH and ions for the enzyme. Wrong buffer can affect cutting.
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If the number of EcoRI sites increases in a DNA sample what happens to expected fragments?
A. fragments की संख्या बढ़ सकती हैNumber of fragments may increase
अधिक cut sites होने पर अधिक fragments बन सकते हैं। यह restriction pattern बदल देता है। / More cut sites can produce more fragments. This changes the restriction pattern.
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If a mutation removes a restriction site what may happen in the gel pattern?
A. एक expected cut नहीं होगा और बड़ा fragment दिख सकता हैOne expected cut will not occur and a larger fragment may appear
restriction site हटने से cut कम हो सकता है। इससे पहले के दो fragments मिलकर बड़ा fragment दिखा सकते हैं। / Loss of a restriction site can reduce cutting. Two previous fragments may appear as one larger fragment.
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If a mutation creates a new restriction site what is possible?
A. नया अतिरिक्त cut और अधिक fragmentsA new extra cut and more fragments
नया restriction site additional cut दे सकता है। इससे gel में fragment pattern बदल सकता है। / A new restriction site can give an additional cut. This can change the fragment pattern in a gel.
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