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Biotechnology Restriction Enzymes MCQ Questions for Class 11 Science

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01 Practice Topic Genetic Engineering 200 MCQs 02 Practice Topic Bioprocess Engineering 300 MCQs 03 Practice Topic Ligase Enzymes 290 MCQs 04 Practice Topic Cloning Vectors 295 MCQs 05 Practice Topic Competent Host 445 MCQs

Restriction Enzymes Practice Questions

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Question 181/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक प्रश्न में पूछा गया कि restriction enzyme random cutter है या specific cutter। सही उत्तर क्या होगा?

A question asks whether a restriction enzyme is a random cutter or specific cutter. What is correct?

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A. specific cutter

Explanation

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Restriction enzymes specific recognition sequences पर cut करते हैं। इसलिए उन्हें specific cutters माना जाता है। / Restriction enzymes cut at specific recognition sequences. Therefore they are considered specific cutters.

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Question 182/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किस scenario में blunt-end ligation sticky-end ligation से कठिन हो सकती है?

In which scenario can blunt-end ligation be harder than sticky-end ligation?

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A. जब complementary overhang मौजूद न होंWhen complementary overhangs are absent

Explanation

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Blunt ends में overhang नहीं होते इसलिए base pairing सहायता नहीं मिलती। इससे ligation तुलनात्मक रूप से कठिन हो सकती है। / Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.

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Question 183/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किस option में restriction digestion और gel electrophoresis का सही क्रम है?

Which option gives the correct order of restriction digestion and gel electrophoresis?

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Correct Answer

A. पहले DNA cut फिर fragments अलगFirst cut DNA then separate fragments

Explanation

Simple Explanation

पहले restriction enzyme DNA काटता है फिर gel में fragments अलग किए जाते हैं। यही प्रयोग का logical order है। / First the restriction enzyme cuts DNA then the fragments are separated in a gel. This is the logical order of the experiment.

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Question 184/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक plasmid map में EcoRI और HindIII sites marked हैं। यह map किस जानकारी के लिए है?

EcoRI and HindIII sites are marked on a plasmid map. What information does this map provide?

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Correct Answer

A. restriction sites की स्थितिPositions of restriction sites

Explanation

Simple Explanation

Plasmid map पर enzyme sites दिखाकर cloning planning की जाती है। इससे cut positions समझ आते हैं। / A plasmid map shows enzyme sites for cloning planning. It helps understand cut positions.

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Question 185/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किस कारण plasmid में multiple cloning site उपयोगी होती है?

Why is a multiple cloning site useful in a plasmid?

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Correct Answer

A. उसमें कई restriction sites होते हैंIt contains many restriction sites

Explanation

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Multiple cloning site में कई restriction sites होने से insert डालने के विकल्प मिलते हैं। इसे cloning flexibility से जोड़ें। / A multiple cloning site provides options for inserting DNA through many restriction sites. Link it with cloning flexibility.

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Question 186/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

अगर insert के दोनों ends same sticky type के हैं तो क्या जोखिम हो सकता है?

If both ends of an insert are the same sticky type what risk may occur?

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A. insert दोनों directions में लग सकता हैInsert may ligate in either direction

Explanation

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Same compatible ends होने पर orientation control कम हो सकता है। Directional cloning इस समस्या को घटाती है। / With same compatible ends orientation control may be reduced. Directional cloning reduces this issue.

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Question 187/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक restriction enzyme DNA के बीच में cut करता है। इसे exonuclease क्यों नहीं कहा जाएगा?

A restriction enzyme cuts within DNA. Why is it not called an exonuclease?

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A. क्योंकि यह अंदर से काटता हैBecause it cuts internally

Explanation

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Restriction enzymes endonucleases हैं क्योंकि वे DNA के अंदर cut करते हैं। Exonuclease ends से nucleotides हटाता है। / Restriction enzymes are endonucleases because they cut within DNA. Exonucleases remove nucleotides from ends.

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Question 188/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

किस उत्तर में restriction enzyme का natural और laboratory role दोनों शामिल हैं?

Which answer includes both natural and laboratory roles of restriction enzymes?

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A. bacteria में defense और lab में DNA cloningDefense in bacteria and DNA cloning in lab

Explanation

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Natural role foreign DNA defense है और lab role DNA manipulation है। यह topic का complete view देता है। / The natural role is defense against foreign DNA and the lab role is DNA manipulation. This gives a complete view of the topic.

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Question 189/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

एक examiner पूछता है कि EcoRI क्यों useful है। सबसे सही कारण क्या है?

An examiner asks why EcoRI is useful. What is the best reason?

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Correct Answer

A. यह specific sequence cut करके sticky ends बना सकता हैIt can cut a specific sequence and make sticky ends

Explanation

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EcoRI की usefulness उसकी specific cutting और sticky ends से जुड़ी है। इसे recombinant DNA से जोड़ें। / The usefulness of EcoRI is linked with specific cutting and sticky ends. Connect it with recombinant DNA.

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Question 190/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 14

restriction enzyme द्वारा cut site predict करने के लिए DNA में क्या देखना होगा?

What must be checked in DNA to predict a restriction enzyme cut site?

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Correct Answer

A. उस enzyme का recognition sequenceRecognition sequence of that enzyme

Explanation

Simple Explanation

Cut site recognition sequence पर निर्भर करता है। इसलिए sequence check करना जरूरी है। / The cut site depends on the recognition sequence. Therefore checking the sequence is necessary.

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