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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 561/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में linker और adapter में common बात क्या है?

What is common between linker and adapter in restriction digestion work?

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A. दोनों cloning में useful DNA ends provide कर सकते हैंBoth can provide useful DNA ends for cloning

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Linkers और adapters छोटे DNA molecules होते हैं। ये desired ends या sites जोड़ने में मदद कर सकते हैं। / Linkers and adapters are small DNA molecules. They can help add desired ends or sites.

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Question 562/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में adapter dimer क्यों unwanted हो सकता है?

Why can adapter dimer be unwanted in restriction digestion work?

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A. वह cloning reaction में background दे सकता हैIt can give background in cloning reaction

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Adapter dimers small ligation products बनाकर cloning को confuse कर सकते हैं। Size selection cleanup helpful है। / Adapter dimers can form small ligation products and confuse cloning. Size selection cleanup is helpful.

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Question 563/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में size selection का उद्देश्य क्या है?

What is the purpose of size selection in restriction digestion?

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A. desired size fragment को चुननाSelecting desired size fragment

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Size selection unwanted fragments से desired DNA अलग करता है। यह cloning accuracy बढ़ाता है। / Size selection separates desired DNA from unwanted fragments. It increases cloning accuracy.

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Question 564/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में magnetic bead cleanup कब useful हो सकता है?

When can magnetic bead cleanup be useful in restriction digestion?

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A. DNA fragments को size या purification के आधार पर recover करने मेंIn recovering DNA fragments by size or purification

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Magnetic beads DNA cleanup और size selection में use हो सकती हैं। Protocol bead ratio पर depend करता है। / Magnetic beads can be used in DNA cleanup and size selection. Protocol depends on bead ratio.

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Question 565/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction fragments की bead cleanup में bead ratio क्यों important है?

Why is bead ratio important in bead cleanup of restriction fragments?

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A. कौन से size fragments bind होंगे यह प्रभावित करता हैIt affects which size fragments bind

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Bead ratio DNA size selection को प्रभावित कर सकता है। सही ratio desired fragment recovery में मदद करता है। / Bead ratio can affect DNA size selection. Correct ratio helps recover the desired fragment.

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Question 566/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digest cleanup में beads को over-dry करने से क्या समस्या हो सकती है?

What problem can occur if beads are over-dried in restriction digest cleanup?

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A. DNA elution कठिन हो सकती हैDNA elution can become difficult

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Over-dried beads DNA release कम कर सकती हैं। Proper drying time important है। / Over-dried beads can reduce DNA release. Proper drying time is important.

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Question 567/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction fragment cleanup में ethanol wash के बाद drying क्यों जरूरी है?

Why is drying necessary after ethanol wash in restriction fragment cleanup?

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A. residual ethanol हटाने के लिएTo remove residual ethanol

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Residual ethanol ligation या digestion inhibit कर सकता है। लेकिन over-drying भी avoid करें। / Residual ethanol can inhibit ligation or digestion. But avoid over-drying too.

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Question 568/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में desalting column किसलिए useful हो सकता है?

Why can a desalting column be useful in restriction digestion?

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A. salts हटाने और buffer exchange के लिएTo remove salts and exchange buffer

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Excess salts next enzyme reactions को affect कर सकते हैं। Desalting reaction compatibility सुधारता है। / Excess salts can affect next enzyme reactions. Desalting improves reaction compatibility.

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Question 569/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digest product में buffer exchange कब जरूरत हो सकती है?

When can buffer exchange be needed for restriction digest product?

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A. next enzyme या ligase buffer incompatible होWhen next enzyme or ligase buffer is incompatible

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Buffer exchange unwanted salts और components हटाकर next reaction के लिए suitable condition देता है। / Buffer exchange removes unwanted salts and components to give suitable condition for next reaction.

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Question 570/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 56

Restriction digestion में DNA end repair कब जरूरी हो सकता है?

When can DNA end repair be needed in restriction digestion work?

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A. जब ends cloning strategy के लिए suitable न होंWhen ends are not suitable for cloning strategy

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End repair blunt या compatible ends बनाने के लिए use हो सकता है। यह cloning method पर निर्भर करता है। / End repair can be used to make blunt or compatible ends. It depends on cloning method.

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