29 results found for "methylation" in all classes.
Dam or Dcm methylation can be related to what?
A. bacterial DNA methylation
कुछ bacterial strains DNA को methylate करते हैं। यह restriction digestion patterns को प्रभावित कर सकता है। / Some bacterial strains methylate DNA. This can affect restriction digestion patterns.
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From where can DNA be prepared to avoid methylation problem?
A. suitable methylation-deficient host strain सेFrom a suitable methylation-deficient host strain
Methylation-deficient host कुछ sensitive enzymes के लिए DNA suitable बना सकता है। Enzyme requirement पहले check करें। / A methylation-deficient host can make DNA suitable for some sensitive enzymes. Check enzyme requirements first.
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Dcm methylation is related to what?
A. cytosine methylation in certain bacterial sequences
Dcm methylation bacterial DNA में cytosine modification से जुड़ी है। कुछ restriction enzymes इससे प्रभावित हो सकते हैं। / Dcm methylation is linked with cytosine modification in bacterial DNA. Some restriction enzymes can be affected by it.
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Dam methylation is related to which base?
A. adenine methylation in certain bacterial sites
Dam methylation bacterial DNA में adenine modification से जुड़ी है। यह कुछ enzyme cutting को प्रभावित कर सकती है। / Dam methylation is linked with adenine modification in bacterial DNA. It can affect cutting by some enzymes.
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How can methylation block be identified in restriction digestion?
A. methylation-sensitive और insensitive enzymes compare करकेBy comparing methylation-sensitive and insensitive enzymes
अगर methylation-sensitive enzyme cut न करे तो methylation cause हो सकता है। Alternative enzyme या host source मदद कर सकता है। / If a methylation-sensitive enzyme does not cut methylation can be a cause. Alternative enzyme or host source can help.
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If a restriction enzyme is methylation-sensitive what should be considered in cloning plan?
A. DNA source और methylation statusDNA source and methylation status
Methylated DNA enzyme cutting रोक सकता है। इसलिए suitable host strain या alternative enzyme चुना जा सकता है। / Methylated DNA can block enzyme cutting. Therefore a suitable host strain or alternative enzyme can be chosen.
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What can be the natural purpose of host DNA methylation?
A. host DNA को own restriction enzyme से बचानाProtecting host DNA from its own restriction enzyme
Host DNA methylated होने पर restriction enzyme उसे नहीं काटता। Foreign unmethylated DNA काटा जा सकता है। / When host DNA is methylated the restriction enzyme does not cut it. Foreign unmethylated DNA can be cut.
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What does a methylation-sensitive enzyme do?
A. methylated site पर cutting बदल सकती हैIts cutting can change at methylated site
Methylation-sensitive enzymes methylated DNA को कम या नहीं काट सकते। यह digestion pattern बदल सकता है। / Methylation-sensitive enzymes may cut methylated DNA poorly or not at all. This can change digestion pattern.
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Why can restriction digest fail due to DNA methylation?
A. methylation enzyme recognition रोक सकती हैMethylation can block enzyme recognition
कुछ enzymes methylated recognition sites नहीं काटते। Methylation sensitivity enzyme choice में देखनी चाहिए। / Some enzymes do not cut methylated recognition sites. Methylation sensitivity should be checked in enzyme choice.
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What effect can DNA methylation have in restriction digestion?
A. enzyme recognition या cutting को प्रभावित कर सकती हैIt can affect enzyme recognition or cutting
कुछ restriction enzymes methylated sites नहीं काटते। इसलिए methylation status digestion result बदल सकता है। / Some restriction enzymes do not cut methylated sites. Therefore methylation status can change digestion results.
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Why can plasmid methylation status matter?
A. Host restriction enzymes DNA recognition को प्रभावित कर सकते हैंHost restriction enzymes can be affected by DNA recognition
Methylation कुछ restriction systems से DNA को बचा सकती है या digestion pattern बदल सकती है। यह host system पर निर्भर करता है। / Methylation can protect DNA from some restriction systems or change digestion pattern. It depends on the host system.
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If a DNA sample has methylation what complication can occur in restriction analysis?
A. कुछ sites cut न होंSome sites may not cut
Methylation कुछ restriction enzymes को cut करने से रोक सकती है। इससे expected bands बदल सकते हैं। / Methylation can prevent some restriction enzymes from cutting. This may change expected bands.
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What may happen in bacteria if foreign DNA lacks methylation?
A. प्रतिबंध एंजाइम उसे काट सकते हैंRestriction enzymes may cut it
मेथिलेशन न होने पर विदेशी डीएनए स्वयं DNA जैसा सुरक्षित नहीं रहता। इसलिए restriction enzymes उसे काट सकते हैं। / Without methylation foreign DNA is not protected like self DNA. Therefore restriction enzymes may cut it.
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Why can some restriction enzymes fail if DNA is methylated?
A. methylation recognition या cutting रोक सकती हैMethylation can block recognition or cutting
कुछ enzymes methylated recognition sites नहीं काटते। इसलिए methylation sensitivity देखना जरूरी है। / Some enzymes do not cut methylated recognition sites. Therefore methylation sensitivity must be checked.
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What does modification show in restriction modification system?
A. host DNA methylation
Modification part host DNA को methylate करके protection देता है। Restriction part foreign DNA काटता है। / The modification part protects host DNA by methylation. The restriction part cuts foreign DNA.
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How does bacterial host protect its own DNA from its restriction enzyme?
A. methylation द्वाराBy methylation
Host DNA methylation own recognition sites को protect कर सकती है। Foreign unmethylated DNA cut हो सकता है। / Host DNA methylation can protect its own recognition sites. Foreign unmethylated DNA can be cut.
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Why is restriction enzyme supplier information checked?
A. buffer temperature methylation sensitivity और heat inactivation जानने के लिएTo know buffer temperature methylation sensitivity and heat inactivation
हर enzyme की working condition अलग हो सकती है। Official data सही reaction setup में मदद करता है। / Each enzyme can have different working conditions. Official data helps correct reaction setup.
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If test DNA does not cut but positive control cuts what can be the reason?
A. test DNA में site absent या methylated हो सकती हैTest DNA may lack site or be methylated
Positive control enzyme activity दिखाता है। Test DNA में recognition site या methylation issue हो सकता है। / Positive control shows enzyme activity. Test DNA may have recognition site or methylation issue.
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What does modification mean in a restriction-modification system?
C. host DNA को methylate करनाMethylating host DNA
Modification usually host DNA methylation से जुड़ी है। इससे host DNA cutting से बच सकता है। / Modification is usually linked with methylation of host DNA. This can protect host DNA from cutting.
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How can bacteria protect their own DNA from restriction enzymes?
B. methylation द्वाराBy methylation
Bacteria अपने recognition sites को methylate कर सकते हैं। इससे own DNA restriction enzyme से सुरक्षित रहता है। / Bacteria can methylate their recognition sites. This protects their own DNA from restriction enzymes.
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If a bacterium cannot methylate its own DNA what risk will occur?
A. अपना DNA restriction enzyme से cut हो सकता हैIts own DNA may be cut by restriction enzyme
Methylation self DNA को restriction enzyme से बचाती है। इसके बिना own DNA भी vulnerable हो सकता है। / Methylation protects self DNA from restriction enzymes. Without it self DNA can become vulnerable.
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If protection is absent in foreign DNA what can a bacterial restriction enzyme do?
A. foreign DNA cut कर सकता हैIt can cut foreign DNA
Foreign DNA methylated न हो तो restriction enzyme उसे पहचानकर cut कर सकता है। यह bacterial defense का हिस्सा है। / If foreign DNA is not methylated the restriction enzyme can recognize and cut it. This is part of bacterial defense.
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Which option gives the correct pair for bacterial DNA protection?
A. methylase और methylationMethylase and methylation
Methylase bacterial DNA को methylate कर सकता है। इससे restriction enzyme अपना DNA नहीं काटता। / Methylase can methylate bacterial DNA. This prevents restriction enzymes from cutting self DNA.
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What may happen if the recognition site is methylated?
A. cutting रुक या घट सकती हैCutting may stop or decrease
Methylation कई restriction enzymes की cutting रोक सकती है। इसी से bacteria अपना DNA बचाते हैं। / Methylation can block cutting by many restriction enzymes. This helps bacteria protect their own DNA.
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What do bacteria do to protect their own DNA from restriction enzymes?
A. मेथिलेशनMethylation
जीवाणु अपने डीएनए को मेथिलेट करके कटने से बचाते हैं। विदेशी डीएनए में ऐसा संरक्षण न हो तो वह कट सकता है। / Bacteria protect their own DNA by methylation. Foreign DNA may be cut if it lacks this protection.
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A bacterium methylates its DNA. What is the benefit against restriction enzymes?
A. अपना DNA कटने से बचता हैIts own DNA is protected from cutting
मेथिलेशन self DNA को पहचानकर कटने से बचा सकता है। यह restriction-modification system का हिस्सा है। / Methylation can protect self DNA from being cut. This is part of the restriction-modification system.
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How may a restriction enzyme act on a methylated recognition site?
A. कटिंग रुक या घट सकती हैCutting may be blocked or reduced
मेथिलेशन कई प्रतिबंध एंजाइमों की कटिंग को रोक सकता है। जीवाणु अपना DNA इसी प्रकार बचाते हैं। / Methylation can block cutting by many restriction enzymes. Bacteria protect their own DNA in this way.
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What is the main role of the modification part in a restriction-modification system?
A. अपने डीएनए की मेथिलेशन से रक्षा करनाProtecting self DNA by methylation
संशोधन भाग अपना डीएनए मेथिलेट करता है। इससे प्रतिबंध एंजाइम अपना डीएनए नहीं काटता। / The modification part methylates self DNA. This prevents the restriction enzyme from cutting self DNA.
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How do bacteria protect their own DNA from restriction enzymes?
B. मेथिलेशन द्वाराBy methylation
जीवाणु अपने डीएनए को मेथिलेशन से सुरक्षित रखते हैं। विदेशी डीएनए मेथिलेटेड न होने पर कट सकता है। / Bacteria protect their own DNA by methylation. Foreign DNA can be cut if it is not methylated.
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