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Biotechnology Isolation of DNA MCQ Questions for Class 11 General

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01 Practice Topic Cutting of DNA 592 MCQs

Isolation of DNA Practice Questions

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Question 211/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA extraction में low yield का एक सामान्य कारण क्या हो सकता है?

What can be a common reason for low yield in DNA extraction?

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A. अपूर्ण cell lysisIncomplete cell lysis

Explanation

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अगर cells पूरी तरह नहीं टूटतीं तो DNA release कम होगा। Lysis step yield के लिए critical है। / If cells are not fully broken DNA release will be low. The lysis step is critical for yield.

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Question 212/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA yield कम हो लेकिन purity अच्छी हो तो क्या अर्थ है?

What does low yield but good purity mean?

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B. DNA साफ है पर मात्रा कम हैDNA is clean but quantity is low

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Purity और yield अलग parameters हैं। अच्छा ratio high concentration की guarantee नहीं देता। / Purity and yield are different parameters. A good ratio does not guarantee high concentration.

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Question 213/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA purity खराब हो लेकिन yield high हो तो क्या करना उचित हो सकता है?

If DNA purity is poor but yield is high what may be appropriate?

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C. additional cleanup करनाPerform additional cleanup

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High yield sample भी impurities के कारण unusable हो सकता है। Cleanup downstream reactions के लिए मदद करता है। / A high-yield sample can still be unusable due to impurities. Cleanup helps downstream reactions.

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Question 214/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA cleanup का मुख्य उद्देश्य क्या है?

What is the main purpose of DNA cleanup?

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D. inhibitors और impurities हटानाRemoving inhibitors and impurities

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Cleanup residual salts phenol protein या ethanol जैसी impurities हटाने में मदद करता है। इससे PCR और digestion बेहतर हो सकते हैं। / Cleanup helps remove residual impurities such as salts phenol protein or ethanol. This can improve PCR and digestion.

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Question 215/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

PCR inhibitors DNA extraction में कहाँ से आ सकते हैं?

Where can PCR inhibitors come from in DNA extraction?

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A. plant compounds salts phenol या ethanol सेFrom plant compounds salts phenol or ethanol

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PCR inhibitors amplification को रोक सकते हैं। Proper cleanup और dilution मदद कर सकते हैं। / PCR inhibitors can stop amplification. Proper cleanup and dilution can help.

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Question 216/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA sample dilute करना कब helpful हो सकता है?

When can diluting a DNA sample be helpful?

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B. inhibitors की effect घटाने या concentration adjust करने के लिएTo reduce inhibitor effect or adjust concentration

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Dilution template amount और inhibitor concentration दोनों adjust कर सकती है। PCR setup में यह practical है। / Dilution can adjust both template amount and inhibitor concentration. It is practical in PCR setup.

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Question 217/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

Restriction digestion के लिए DNA sample कैसा होना चाहिए?

What kind of DNA sample is needed for restriction digestion?

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C. enzyme-compatible clean DNA

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Restriction enzymes impurities से inhibit हो सकते हैं। Clean DNA और suitable buffer जरूरी हैं। / Restriction enzymes can be inhibited by impurities. Clean DNA and suitable buffer are necessary.

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Question 218/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA isolation के बाद cloning के लिए सबसे जरूरी quality क्या है?

What is the most important quality for cloning after DNA isolation?

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D. usable purity और intact DNAUsable purity and intact DNA

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Cloning के लिए DNA contaminants से मुक्त और sufficiently intact होना चाहिए। Poor quality cloning failure दे सकती है। / For cloning DNA should be free from contaminants and sufficiently intact. Poor quality can cause cloning failure.

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Question 219/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA concentration बहुत अधिक हो तो PCR में क्या करना चाहिए?

If DNA concentration is too high what should be done for PCR?

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A. proper dilution बनानाMake a proper dilution

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PCR में template amount optimized होना चाहिए। Dilution suitable concentration देता है। / Template amount should be optimized in PCR. Dilution gives a suitable concentration.

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Question 220/294 Easy Biotechnology Processes of Recombinant DNA Technology Isolation of DNA Class 11 Level 43

DNA concentration बहुत कम हो तो कौन सा issue आ सकता है?

What issue can occur if DNA concentration is too low?

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B. DNA हमेशा बेहतर होगाDNA will always be better

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बहुत कम template downstream reactions में weak signal दे सकता है। Concentration check important है। / Too little template can give weak signal in downstream reactions. Concentration check is important.

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