Muft Shiksha™ एक 100% Free Education Portal है 🇮🇳, जिसका उद्देश्य Class 9–12 के हर विद्यार्थी तक High-Quality Education को पूरी तरह मुफ्त पहुँचाना है। 🇮🇳 हम मानते हैं कि अच्छी शिक्षा किसी student की आर्थिक स्थिति पर निर्भर नहीं होनी चाहिए। 🇮🇳 हर विद्यार्थी को वही Quality Study Material, MCQs, Quizzes, Exam Preparation, Concept-Based Learning और Bilingual Support मिलना चाहिए, जो आमतौर पर महंगी Coaching या Premium Platforms में मिलता है। Muft Shiksha™ 🇮🇳 इसी सोच के साथ बनाया गया है
In Class 11 Biotechnology, under the chapter Principles of Biotechnology, students learn how a competent host enables the introduction and maintenance of recombinant DNA. The topic explains why host cells such as bacteria are used in genetic engineering, how chemical treatment or other methods can make cells capable of taking up foreign DNA, and how transformed cells are identified, selected, and multiplied for producing a desired gene product.
Easy · Level 8 · 25 questions
TOPIC PRACTICE
Quiz this set
Up to 25 questions from this page. Select your focus, then start.
25 questions
Choose questions
View options
When two plasmids must be maintained together
When there is no DNA
When plate is dry
When host is absent
View options
Which hosts can maintain a vector
Color range of host
Protein taste range
Water range
View options
It can work in many host species
It works only in water
It digests protein
It has no DNA
View options
It works only in some hosts
It is always universal
It makes water
It makes host pigment
View options
For marker expression and selection to work
To make DNA water
To erase host
To cut protein
View options
Host machinery can recognize it
Host always dies
DNA disappears
Marker burns
View options
Transcription machinery is different
Mammalian cells have no DNA
Bacterial promoter is water
Host has no nucleus
View options
To select transformed plant cells
To erase DNA
To make host bacteria
To dry gel
View options
Using a nutrient requirement in selection
Cutting DNA
Making the host water
Coloring protein
View options
Mammalian cells often grow in culture wells
Mammalian cells always form agar colonies
Mammalian cells have no DNA
Mammalian cells are only water
View options
Protein purification can become easier
DNA uptake stops
Host becomes water
Marker disappears
View options
Insoluble protein aggregates
Perfect DNA uptake only
No plasmid always
Water crystals
View options
Identifying and purifying recombinant protein
Stopping DNA uptake
Killing host
Making antibiotic
View options
Spread of resistance gene must be prevented
It makes water
It digests protein
It makes host pigment
View options
To safely inactivate modified live cells
To make DNA sweet
To freeze host
To stain water
View options
To track strain plasmid and result
To track water color
To track host smell
To track fat taste
View options
Wrong strain can give wrong result
Every strain is always same
Strain has no DNA
Strain is only water
View options
Wrong plasmid will not give desired construct
Plasmid is always water
Identity never matters
Host has no DNA
View options
For future use and repeat experiments
To destroy DNA
To make host water
To digest protein
View options
Host choice depends on cloning expression safety and selection
Only water matters
Only starch digestion matters
No DNA is involved
View options
Suitable cell that takes DNA and maintains vector
Only enzyme that digests starch
Pigment that makes light
Mineral that forms bone
View options
More transformants can be obtained
DNA will always disappear
Host becomes water
Protein digestion increases
View options
Maintaining vectors with different DNA fragments
Freezing water
Digesting protein
Coloring agar
View options
To keep vectors carrying genome fragments
To color protein
To burn host
To eat agar
View options
cDNA-containing recombinant vectors
Only water droplets
Protein crystals
Soil particles
Question 1EasyLevel 8
When is plasmid compatibility group important?Plasmid compatibility group कब important होता है?
Correct answer:सही उत्तर:A
Plasmids of the same compatibility group may not remain stable together. This is important in multiple plasmid systems.Same compatibility group के plasmids साथ stable नहीं रह सकते। Multiple plasmid systems में यह जरूरी point है।
What does host range mean?Host range का अर्थ क्या है?
Correct answer:सही उत्तर:A
Host range tells in which hosts a vector can work. Broad host range vectors are useful in more hosts.Host range बताता है कि vector किन hosts में काम कर सकता है। Broad host range vectors अधिक hosts में उपयोगी होते हैं।
What is the benefit of a broad host range vector?Broad host range vector का लाभ क्या है?
Correct answer:सही उत्तर:A
A broad host range vector can replicate in many bacterial hosts. It helps in versatile cloning.Broad host range vector कई bacterial hosts में replicate हो सकता है। यह versatile cloning में मदद करता है।
What is a limitation of a narrow host range vector?Narrow host range vector की limitation क्या है?
Correct answer:सही उत्तर:A
A narrow host range vector does not replicate in every host. Therefore host selection must be careful.Narrow host range vector हर host में replicate नहीं करता। इसलिए host selection ध्यान से करनी होती है।
Why should a selectable marker be host-compatible?Selectable marker host-compatible क्यों होना चाहिए?
Correct answer:सही उत्तर:A
The marker should express in the host and match the selection condition. Only then transformants are selected.Marker host में express होना चाहिए और selection condition से match होना चाहिए। तभी transformants चुने जाते हैं।
Why should a promoter be host-compatible?Promoter host-compatible क्यों होना चाहिए?
Correct answer:सही उत्तर:A
The host transcription machinery should recognize the promoter. Otherwise gene expression can be weak or absent.Promoter को host transcription machinery पहचाननी चाहिए। Otherwise gene expression weak या absent हो सकता है।
Why does a bacterial promoter not always work in a mammalian host?Bacterial promoter mammalian host में हमेशा क्यों काम नहीं करता?
Correct answer:सही उत्तर:A
Different hosts recognize different promoters. Therefore expression vector elements can be host-specific.Different hosts अलग promoters recognize करते हैं। इसलिए expression vector के elements host-specific हो सकते हैं।
Why can a herbicide marker be useful in a plant host?Plant host में herbicide marker किसलिए useful हो सकता है?
Correct answer:सही उत्तर:A
In plant transformation the selection marker is chosen according to host and culture condition. Herbicide resistance can also help selection.Plant transformation में selection marker host और culture condition के अनुसार चुना जाता है। Herbicide resistance भी selection में मदद कर सकता है।
What is the simple meaning of an auxotrophic marker?Auxotrophic marker का सरल अर्थ क्या है?
Correct answer:सही उत्तर:A
The governing concept is nutritional selection based on auxotrophy. An auxotrophic host has a mutation that prevents it from synthesizing a particular essential compound, so it cannot grow on a medium lacking that nutrient. If a vector carries a functional gene that complements the defect, transformed cells regain the ability to grow under the selective condition. Consequently, the nutrient requirement becomes a convenient marker for identifying transformants. Option A is correct. DNA cutting is performed by restriction enzymes, protein staining is an analytical method, and making water is unrelated to marker function; therefore B, C, and D are wrong.इसका मुख्य सिद्धान्त auxotrophy पर आधारित nutritional selection है। Auxotrophic host में ऐसा mutation होता है, जिसके कारण वह किसी आवश्यक compound का निर्माण नहीं कर पाता और उस nutrient से रहित माध्यम पर बढ़ नहीं सकता। यदि vector में उस कमी को पूरा करने वाला functional gene मौजूद हो, तो transformed कोशिकाएँ selective medium पर फिर से बढ़ने लगती हैं। इस प्रकार nutrient requirement transformants की पहचान के लिए marker की तरह काम करती है। इसलिए A सही है। DNA काटने का कार्य restriction enzymes करते हैं, protein staining अलग विश्लेषणात्मक विधि है और पानी बनाना marker के कार्य से असंबंधित है; अतः B, C और D गलत हैं।
How can mammalian cell screening differ from bacterial colony screening?Mammalian cell screening bacterial colony screening से कैसे अलग हो सकती है?
Correct answer:सही उत्तर:A
Mammalian cells do not form simple agar plate colonies like bacteria. Their culture system is different.Mammalian cells bacterial colonies की तरह simple agar plate colonies नहीं बनातीं। Their culture system is different.
What can be a benefit of a secretory expression system?Secretory expression system का लाभ क्या हो सकता है?
Correct answer:सही उत्तर:A
If protein is secreted into the medium purification can become easier. This is a useful point in expression design.यदि protein medium में secreted हो तो purification आसान हो सकती है। यह expression design में useful point है।
Inclusion bodies can indicate what?Inclusion bodies किसका संकेत हो सकती हैं?
Correct answer:सही उत्तर:A
Some expressed proteins can form insoluble aggregates. Such aggregates are called inclusion bodies.कुछ expressed proteins insoluble aggregates बना सकते हैं। ऐसे aggregates को inclusion bodies कहते हैं।
What is the main use of a protein purification tag?Protein purification tag का मुख्य उपयोग क्या है?
Correct answer:सही उत्तर:A
Tags like His-tag help in protein purification and detection. This is common in expression vector design.His-tag जैसे tags protein purification और detection में मदद करते हैं। यह expression vector design में common है।
Why can an antibiotic resistance marker be a safety concern?Antibiotic resistance marker safety concern क्यों हो सकता है?
Correct answer:सही उत्तर:A
Antibiotic resistance markers should be handled with containment. Biosafety rules manage this risk.Antibiotic resistance markers को containment के साथ handle करना चाहिए। Biosafety rules इस risk को manage करते हैं।
Proper disposal of modified cells and contaminated materials is necessary for biosafety. Link it with lab safety.Modified cells और contaminated materials का proper disposal biosafety के लिए जरूरी है। इसे lab safety से जोड़ें।
Why is record keeping necessary in competent host experiments?Record keeping competent host प्रयोग में क्यों जरूरी है?
Correct answer:सही उत्तर:A
Records maintain traceability of strain plasmid marker and result. This improves reproducibility.Records strain plasmid marker और result की traceability रखते हैं। इससे reproducibility बेहतर होती है।
Why is it important to confirm strain identity?Strain identity confirm करना क्यों जरूरी है?
Correct answer:सही उत्तर:A
Different strains have different properties. A wrong strain can change cloning or expression results.Different strains की properties अलग होती हैं। गलत strain cloning या expression result बदल सकता है।
Why is it important to confirm plasmid identity?Plasmid identity confirm करना क्यों जरूरी है?
Correct answer:सही उत्तर:A
A wrong plasmid may lack the desired insert or marker. Plasmid verification improves experiment reliability.Wrong plasmid में desired insert या marker absent हो सकता है। Plasmid verification experiment reliability बढ़ाता है।
Why is final clone storage done in competent host work?Competent host में final clone storage क्यों किया जाता है?
Correct answer:सही उत्तर:A
A confirmed clone can be preserved in stock. This provides the same clone for future experiments.Confirmed clone को stock में preserve किया जा सकता है। इससे future experiments में same clone मिल जाता है।
What is the safest revision point of Level 33?Level 33 का सबसे सुरक्षित revision point क्या है?
Correct answer:सही उत्तर:A
Competent host selection is based on experiment goal. Remember compatibility selection and biosafety together.Competent host selection experiment goal पर आधारित होता है। Compatibility selection और biosafety को साथ याद रखें।
What is the exam-level summary of a competent host?Competent host का exam-level summary क्या है?
Correct answer:सही उत्तर:A
A competent host helps in DNA uptake and vector maintenance. This is the core of the transformation workflow.Competent host DNA uptake और vector maintenance दोनों में मदद करता है। यही transformation workflow का core है।
What is the benefit of high transformation efficiency in a competent host?Competent host में transformation efficiency अधिक होने का लाभ क्या है?
Correct answer:सही उत्तर:A
High-efficiency cells can give more colonies even with low DNA. This is useful in library construction.High efficiency cells कम डीएनए से भी अधिक colonies दे सकती हैं। यह library construction में उपयोगी है।
What is the role of a competent host in library construction?Library construction में competent host की भूमिका क्या है?
Correct answer:सही उत्तर:A
In a library many DNA fragments are cloned into vectors. A competent host helps take up and maintain them.Library में अनेक DNA fragments vectors में clone होते हैं। Competent host उन्हें uptake और maintain करने में मदद करता है।
Why is a competent host needed in a genomic library?Genomic library में competent host किसलिए जरूरी है?
Correct answer:सही उत्तर:A
In a genomic library genome fragments are kept in vectors. A competent host maintains these recombinant vectors.Genomic library में genome fragments vectors में रखे जाते हैं। Competent host इन recombinant vectors को maintain करता है।
What does a competent host maintain in a cDNA library?cDNA library में competent host क्या maintain करता है?
Correct answer:सही उत्तर:A
cDNA fragments are cloned into vectors. The competent host maintains those recombinant vectors.cDNA fragments vectors में clone किए जाते हैं। Competent host उन recombinant vectors को maintain करता है।
Google Analytics helps us understand site usage. Microsoft Clarity records usage interactions after analytics consent. Google may send limited cookie-free signals before your choice. The Live Visitors widget operates independently of this analytics choice; see the privacy policy for its provider and fallback details. Essential site features work without analytics cookies. You can change your choice later in Privacy choices. Privacy policy
