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Subjects

Biotechnology

Competent Host

In Class 11 Biotechnology, under the chapter Principles of Biotechnology, students learn how a competent host enables the introduction and maintenance of recombinant DNA. The topic explains why host cells such as bacteria are used in genetic engineering, how chemical treatment or other methods can make cells capable of taking up foreign DNA, and how transformed cells are identified, selected, and multiplied for producing a desired gene product.

Easy · Level 8 · 25 questions

Practice questions

01When is plasmid compatibility group important?

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02What does host range mean?

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03What is the benefit of a broad host range vector?

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04What is a limitation of a narrow host range vector?

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05Why should a selectable marker be host-compatible?

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06Why should a promoter be host-compatible?

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07Why does a bacterial promoter not always work in a mammalian host?

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08Why can a herbicide marker be useful in a plant host?

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09What is the simple meaning of an auxotrophic marker?

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10How can mammalian cell screening differ from bacterial colony screening?

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11What can be a benefit of a secretory expression system?

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12Inclusion bodies can indicate what?

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13What is the main use of a protein purification tag?

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14Why can an antibiotic resistance marker be a safety concern?

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15Why is competent host waste disposal necessary?

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16Why is record keeping necessary in competent host experiments?

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17Why is it important to confirm strain identity?

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18Why is it important to confirm plasmid identity?

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19Why is final clone storage done in competent host work?

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20What is the safest revision point of Level 33?

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21What is the exam-level summary of a competent host?

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22What is the benefit of high transformation efficiency in a competent host?

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23What is the role of a competent host in library construction?

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24Why is a competent host needed in a genomic library?

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25What does a competent host maintain in a cDNA library?

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