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Biotechnology

Bioprocess Engineering

Bioprocess Engineering introduces Class 11 students to the methods used to design, control, and scale up biological processes for making useful products. Within the Biotechnology chapter “Principles of Biotechnology,” the topic connects microbial growth and fermentation with bioreactors, nutrient and oxygen control, temperature and pH regulation, sterility, and product recovery. Students see how laboratory discoveries are developed into reliable processes for producing medicines, enzymes, vaccines, food products, and other bio-based materials.

Easy · Level 13 · 1 questions

Practice questions

01What is the role of DNA ligase in making a recombinant vector?

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Answer and explanation

Correct answer: B. Joining the insert and vector

DNA ligase is the enzyme that seals breaks in the sugar-phosphate backbone of DNA. During recombinant-vector construction, the vector and the desired insert are first cut to produce compatible ends, often sticky ends. Base pairing can hold these ends together temporarily, but ligase forms the covalent phosphodiester bonds that permanently join the fragments and produce a continuous recombinant DNA molecule. Option B is correct. Restriction enzymes perform the cutting step; ligase does not randomly cut DNA. Colouring a host and converting a vector into fat are unrelated biological actions and cannot create a recombinant molecule.

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Bioprocess Engineering

Practice questions

01What is the role of DNA ligase in making a recombinant vector?रिकॉम्बिनेंट वेक्टर बनाने में डीएनए लाइगेज की क्या भूमिका होती है?

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01What is the role of DNA ligase in making a recombinant vector?