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A. cell competence और DNA quality/Cell competence and DNA quality
Step 1
Concept
Competence and clean DNA both help transformation success. Poor quality DNA can reduce efficiency.
Step 2
Why this answer is correct
The correct answer is A. cell competence और DNA quality / Cell competence and DNA quality. Competence and clean DNA both help transformation success. Poor quality DNA can reduce efficiency.
Step 3
Exam Tip
Competence और clean DNA दोनों transformation success में मदद करते हैं। Poor quality DNA efficiency घटा सकता है।
A. Uptake या survival घट सकता है/Uptake or survival can decrease
Step 1
Concept
Impurities can stress competent cells. Clean DNA helps better transformation.
Step 2
Why this answer is correct
The correct answer is A. Uptake या survival घट सकता है / Uptake or survival can decrease. Impurities can stress competent cells. Clean DNA helps better transformation.
Step 3
Exam Tip
Impurities competent cells को stress दे सकती हैं। Clean DNA better transformation में मदद करता है।
Too much DNA or impurities can stress cells. An optimal DNA amount is better.
Step 2
Why this answer is correct
The correct answer is A. Cells stress हो सकती हैं / Cells can be stressed. Too much DNA or impurities can stress cells. An optimal DNA amount is better.
Step 3
Exam Tip
बहुत अधिक DNA या impurities cells पर stress डाल सकते हैं। Optimal DNA amount better होता है।
Actively growing cells can respond better to competence treatment. Overgrown culture can reduce efficiency.
Step 2
Why this answer is correct
The correct answer is A. Active exponential phase. Actively growing cells can respond better to competence treatment. Overgrown culture can reduce efficiency.
Step 3
Exam Tip
Active growing cells competence treatment के लिए better respond कर सकती हैं। Overgrown culture efficiency घटा सकता है।
A. Transformation efficiency कम हो सकती है/Transformation efficiency may decrease
Step 1
Concept
Cells in an overgrown culture can be less healthy. This can affect both competence and viability.
Step 2
Why this answer is correct
The correct answer is A. Transformation efficiency कम हो सकती है / Transformation efficiency may decrease. Cells in an overgrown culture can be less healthy. This can affect both competence and viability.
Step 3
Exam Tip
Overgrown culture में cells less healthy हो सकती हैं। इससे competence और viability दोनों प्रभावित हो सकते हैं।
A. गलत colonies और गलत interpretation मिल सकती है/Wrong colonies and wrong interpretation may occur
Step 1
Concept
Contamination makes results unreliable. Aseptic technique and control plates are necessary.
Step 2
Why this answer is correct
The correct answer is A. गलत colonies और गलत interpretation मिल सकती है / Wrong colonies and wrong interpretation may occur. Contamination makes results unreliable. Aseptic technique and control plates are necessary.
Step 3
Exam Tip
Contamination result को unreliable बना देती है। Aseptic technique और control plates जरूरी हैं।
A. Mixed growth या variable clone behavior/Mixed growth or variable clone behavior
Step 1
Concept
Colony size can indicate contamination or clone growth differences. Confirmation tests remain necessary.
Step 2
Why this answer is correct
The correct answer is A. Mixed growth या variable clone behavior / Mixed growth or variable clone behavior. Colony size can indicate contamination or clone growth differences. Confirmation tests remain necessary.
Step 3
Exam Tip
Colony size contamination या clone growth difference का संकेत दे सकती है। Confirmation tests जरूरी रहते हैं।
A. हर colony desired recombinant है/Every colony is a desired recombinant
Step 1
Concept
A high colony count can include empty vectors or background. Screening confirms recombinant status.
Step 2
Why this answer is correct
The correct answer is A. हर colony desired recombinant है / Every colony is a desired recombinant. A high colony count can include empty vectors or background. Screening confirms recombinant status.
Step 3
Exam Tip
High colony count में empty vector या background भी हो सकते हैं। Screening recombinant status confirm करती है।
A. Correct gene expression या construct design के लिए/For correct gene expression or construct design
Step 1
Concept
Wrong insert orientation can affect expression or function. Orientation can be checked by PCR or sequencing.
Step 2
Why this answer is correct
The correct answer is A. Correct gene expression या construct design के लिए / For correct gene expression or construct design. Wrong insert orientation can affect expression or function. Orientation can be checked by PCR or sequencing.
Step 3
Exam Tip
Insert wrong orientation में expression या function प्रभावित हो सकता है। Orientation PCR या sequencing से check हो सकती है।
A. Insert सही orientation में आने की संभावना बढ़ती है/It increases the chance of correct insert orientation
Step 1
Concept
Directional cloning helps control insert orientation. This can reduce wrong clones.
Step 2
Why this answer is correct
The correct answer is A. Insert सही orientation में आने की संभावना बढ़ती है / It increases the chance of correct insert orientation. Directional cloning helps control insert orientation. This can reduce wrong clones.
Step 3
Exam Tip
Directional cloning insert orientation control में मदद करती है। इससे wrong clones कम हो सकते हैं।
A. यह marker के कारण select हो सकता है पर insert नहीं रखता/It can be selected due to marker but lacks insert
Step 1
Concept
A self-ligated vector can give false positives. Insert screening helps identify it.
Step 2
Why this answer is correct
The correct answer is A. यह marker के कारण select हो सकता है पर insert नहीं रखता / It can be selected due to marker but lacks insert. A self-ligated vector can give false positives. Insert screening helps identify it.
Step 3
Exam Tip
Self-ligated vector false positives दे सकता है। Insert screening इसे पहचानने में मदद करती है।
A. Self-ligation कम करने के लिए/To reduce self-ligation
Step 1
Concept
Dephosphorylation can reduce vector self-ligation. This can increase the chance of recombinant colonies.
Step 2
Why this answer is correct
The correct answer is A. Self-ligation कम करने के लिए / To reduce self-ligation. Dephosphorylation can reduce vector self-ligation. This can increase the chance of recombinant colonies.
Step 3
Exam Tip
Dephosphorylation vector self-ligation को घटा सकती है। इससे recombinant colonies की संभावना बढ़ सकती है।
A. Recombinant colony frequency बदल सकती है/Recombinant colony frequency can change
Step 1
Concept
An incorrect ratio can give self-ligation or low recombinant yield. A balanced ratio helps cloning success.
Step 2
Why this answer is correct
The correct answer is A. Recombinant colony frequency बदल सकती है / Recombinant colony frequency can change. An incorrect ratio can give self-ligation or low recombinant yield. A balanced ratio helps cloning success.
Step 3
Exam Tip
Incorrect ratio self-ligation या low recombinant yield दे सकता है। Balanced ratio cloning success में मदद करता है।
A. Salts और enzymes cells को affect कर सकते हैं/Salts and enzymes can affect cells
Step 1
Concept
A ligation mix can contain salts or enzymes. In some cases clean-up can improve transformation efficiency.
Step 2
Why this answer is correct
The correct answer is A. Salts और enzymes cells को affect कर सकते हैं / Salts and enzymes can affect cells. A ligation mix can contain salts or enzymes. In some cases clean-up can improve transformation efficiency.
Step 3
Exam Tip
Ligation mix में salts या enzymes हो सकते हैं। कुछ cases में clean-up transformation efficiency सुधार सकता है।
A. यह transformants का estimate देता है/It gives an estimate of transformants
Step 1
Concept
Counting colonies gives an estimate of transformation success. Compare with controls.
Step 2
Why this answer is correct
The correct answer is A. यह transformants का estimate देता है / It gives an estimate of transformants. Counting colonies gives an estimate of transformation success. Compare with controls.
Step 3
Exam Tip
Colonies count करके transformation success का अनुमान मिलता है। Comparison controls के साथ करें।
A. Transformation efficiency कम हो सकती है/Transformation efficiency can decrease
Step 1
Concept
Large plasmids can transform less efficiently than small plasmids. This is a practical factor.
Step 2
Why this answer is correct
The correct answer is A. Transformation efficiency कम हो सकती है / Transformation efficiency can decrease. Large plasmids can transform less efficiently than small plasmids. This is a practical factor.
Step 3
Exam Tip
Large plasmids छोटे plasmids की तुलना में less efficient transform हो सकते हैं। यह practical factor है।
A small clean circular plasmid can generally transform better. Size and purity are important factors.
Step 2
Why this answer is correct
The correct answer is A. Small clean circular plasmid. A small clean circular plasmid can generally transform better. Size and purity are important factors.
Step 3
Exam Tip
Small clean circular plasmid generally better transform हो सकता है। Size और purity important factors हैं।
A. Plasmid stability या DNA quality बदल सकती है/Plasmid stability or DNA quality can change
Step 1
Concept
Host mutations can affect recombination restriction or nuclease activity. Therefore strain choice is important.
Step 2
Why this answer is correct
The correct answer is A. Plasmid stability या DNA quality बदल सकती है / Plasmid stability or DNA quality can change. Host mutations can affect recombination restriction or nuclease activity. Therefore strain choice is important.
Step 3
Exam Tip
Host mutations recombination restriction या nuclease activity को प्रभावित कर सकती हैं। इसलिए strain choice important है।
A. Cloning strain DNA maintenance के लिए और expression strain protein production के लिए optimized हो सकता है/Cloning strain can be optimized for DNA maintenance and expression strain for protein production
Step 1
Concept
Cloning and expression goals are different. The host strain is chosen according to that goal.
Step 2
Why this answer is correct
The correct answer is A. Cloning strain DNA maintenance के लिए और expression strain protein production के लिए optimized हो सकता है / Cloning strain can be optimized for DNA maintenance and expression strain for protein production. Cloning and expression goals are different. The host strain is chosen according to that goal.
Step 3
Exam Tip
Cloning और expression goals अलग होते हैं। Host strain उसी goal के अनुसार चुनी जाती है।
A. Codon optimization या suitable strain से/By codon optimization or suitable strain
Step 1
Concept
Rare codons can slow translation. An optimized gene or special expression strain can help.
Step 2
Why this answer is correct
The correct answer is A. Codon optimization या suitable strain से / By codon optimization or suitable strain. Rare codons can slow translation. An optimized gene or special expression strain can help.
Step 3
Exam Tip
Rare codons translation slow कर सकते हैं। Optimized gene या special expression strain मदद कर सकता है।
A. Toxic protein बिना induction बन सकता है/Toxic protein can be produced without induction
Step 1
Concept
A leaky promoter can express a toxic gene unwantedly. Tight regulation is better for toxic proteins.
Step 2
Why this answer is correct
The correct answer is A. Toxic protein बिना induction बन सकता है / Toxic protein can be produced without induction. A leaky promoter can express a toxic gene unwantedly. Tight regulation is better for toxic proteins.
Step 3
Exam Tip
Leaky promoter toxic gene को unwanted express कर सकता है। Tight regulation toxic proteins के लिए बेहतर है।
A. Host growth कम हो सकती है/Host growth can decrease
Step 1
Concept
Too early induction can burden cells. Induction at the proper growth stage can give better yield.
Step 2
Why this answer is correct
The correct answer is A. Host growth कम हो सकती है / Host growth can decrease. Too early induction can burden cells. Induction at the proper growth stage can give better yield.
Step 3
Exam Tip
Too early induction cells पर burden डाल सकती है। Proper growth stage पर induction बेहतर yield दे सकती है।
A. Lower temperature और folding support/Lower temperature and folding support
Step 1
Concept
Lower temperature and chaperones can help folding. This can increase soluble protein yield.
Step 2
Why this answer is correct
The correct answer is A. Lower temperature और folding support / Lower temperature and folding support. Lower temperature and chaperones can help folding. This can increase soluble protein yield.
Step 3
Exam Tip
Lower temperature और chaperones folding में मदद कर सकते हैं। इससे soluble protein yield बढ़ सकती है।
A. कुछ proteins की folding या disulfide formation में/In folding or disulfide formation of some proteins
Step 1
Concept
The periplasm environment can help folding of some proteins. It is an expression strategy.
Step 2
Why this answer is correct
The correct answer is A. कुछ proteins की folding या disulfide formation में / In folding or disulfide formation of some proteins. The periplasm environment can help folding of some proteins. It is an expression strategy.
Step 3
Exam Tip
Periplasm का environment कुछ proteins के folding में मदद कर सकता है। यह expression strategy है।
A. Purification आसान हो सकती है/Purification can be easier
Step 1
Concept
If protein is secreted into the medium cell lysis may be less needed. This can make purification easier.
Step 2
Why this answer is correct
The correct answer is A. Purification आसान हो सकती है / Purification can be easier. If protein is secreted into the medium cell lysis may be less needed. This can make purification easier.
Step 3
Exam Tip
Protein medium में secreted हो तो cell lysis कम जरूरत हो सकती है। इससे purification आसान हो सकती है।
A. Detection और purification आसान करने के लिए/To make detection and purification easier
Step 1
Concept
A protein tag helps identify and purify the recombinant product. His tag is a common example.
Step 2
Why this answer is correct
The correct answer is A. Detection और purification आसान करने के लिए / To make detection and purification easier. A protein tag helps identify and purify the recombinant product. His tag is a common example.
Step 3
Exam Tip
Protein tag recombinant product को identify और purify करने में मदद करता है। His tag common example है।
A. Reporter signal देता है और marker selection देता है/Reporter gives signal and marker gives selection
Step 1
Concept
A reporter can give visible signal while a selectable marker gives growth selection. Both have different roles in screening strategy.
Step 2
Why this answer is correct
The correct answer is A. Reporter signal देता है और marker selection देता है / Reporter gives signal and marker gives selection. A reporter can give visible signal while a selectable marker gives growth selection. Both have different roles in screening strategy.
Step 3
Exam Tip
Reporter visible signal दे सकता है जबकि selectable marker growth selection देता है। दोनों screening strategy में अलग roles रखते हैं।
A. Fluorescence से expression signal मिलता है/Fluorescence gives an expression signal
Step 1
Concept
GFP can give a fluorescence signal. It is a common example of a reporter gene.
Step 2
Why this answer is correct
The correct answer is A. Fluorescence से expression signal मिलता है / Fluorescence gives an expression signal. GFP can give a fluorescence signal. It is a common example of a reporter gene.
Step 3
Exam Tip
GFP fluorescence signal दे सकता है। यह reporter gene का common example है।
A. Nutrient requirement को restore करना/Restoring a nutrient requirement
Step 1
Concept
Auxotrophic yeast does not grow without a nutrient. A marker can restore that growth ability.
Step 2
Why this answer is correct
The correct answer is A. Nutrient requirement को restore करना / Restoring a nutrient requirement. Auxotrophic yeast does not grow without a nutrient. A marker can restore that growth ability.
Step 3
Exam Tip
Auxotrophic yeast किसी nutrient के बिना grow नहीं करता। Marker उस growth ability को restore कर सकता है।
A. Host tissue और culture condition/Host tissue and culture condition
Step 1
Concept
A plant selection marker system should match tissue culture conditions. Only then transformed cells can be selected.
Step 2
Why this answer is correct
The correct answer is A. Host tissue और culture condition / Host tissue and culture condition. A plant selection marker system should match tissue culture conditions. Only then transformed cells can be selected.
Step 3
Exam Tip
Plant selection marker system tissue culture conditions से match होना चाहिए। तभी transformed cells चुनी जा सकती हैं।
A. Vector लेने वाली cells चुनने के लिए/To select cells that received the vector
Step 1
Concept
Selection helps identify and enrich transfected cells. The principle is similar to bacterial selection.
Step 2
Why this answer is correct
The correct answer is A. Vector लेने वाली cells चुनने के लिए / To select cells that received the vector. Selection helps identify and enrich transfected cells. The principle is similar to bacterial selection.
Step 3
Exam Tip
Selection transfected cells को identify और enrich करने में मदद करती है। Principle bacterial selection जैसा ही है।
A. DNA direct cell या nucleus में दिया जाता है/DNA is delivered directly into cell or nucleus
Step 1
Concept
In microinjection DNA is delivered directly. It is different from bacterial chemical transformation.
Step 2
Why this answer is correct
The correct answer is A. DNA direct cell या nucleus में दिया जाता है / DNA is delivered directly into cell or nucleus. In microinjection DNA is delivered directly. It is different from bacterial chemical transformation.
Step 3
Exam Tip
Microinjection में DNA को directly deliver किया जाता है। यह bacterial chemical transformation से अलग delivery method है।
A protoplast is a plant cell with its cell wall removed. This can make DNA entry easier.
Step 2
Why this answer is correct
The correct answer is A. Cell wall हट जाती है / Cell wall is removed. A protoplast is a plant cell with its cell wall removed. This can make DNA entry easier.
Step 3
Exam Tip
Protoplast plant cell है जिसकी cell wall हटाई गई होती है। इससे DNA entry आसान हो सकती है।
A. Original clones को फिर से recover करने के लिए/To recover original clones again
Step 1
Concept
After getting results from screening plates the desired colony can be picked from the master plate. It preserves clone identity.
Step 2
Why this answer is correct
The correct answer is A. Original clones को फिर से recover करने के लिए / To recover original clones again. After getting results from screening plates the desired colony can be picked from the master plate. It preserves clone identity.
Step 3
Exam Tip
Screening plates से result मिलने के बाद master plate से desired colony pick की जा सकती है। यह clone identity बचाती है।
A. Colony pattern की identity बनाए रखना/Maintaining identity of colony pattern
Step 1
Concept
Replica plating allows the same colony arrangement to be tested on different plates. It is useful in screening.
Step 2
Why this answer is correct
The correct answer is A. Colony pattern की identity बनाए रखना / Maintaining identity of colony pattern. Replica plating allows the same colony arrangement to be tested on different plates. It is useful in screening.
Step 3
Exam Tip
Replica plating same colony arrangement को different plates पर test करने देती है। यह screening में useful है।
A. Complementary sequence वाले clone को खोजने के लिए/To find a clone with complementary sequence
Step 1
Concept
A probe binds complementarily to the target DNA sequence. This can identify the desired clone.
Step 2
Why this answer is correct
The correct answer is A. Complementary sequence वाले clone को खोजने के लिए / To find a clone with complementary sequence. A probe binds complementarily to the target DNA sequence. This can identify the desired clone.
Step 3
Exam Tip
Probe target DNA sequence से complementary binding करता है। इससे desired clone identify हो सकता है।
A. Recombinant vectors maintain करना/Maintaining recombinant vectors
Step 1
Concept
In both libraries DNA fragments are cloned into vectors. The competent host maintains these vectors.
Step 2
Why this answer is correct
The correct answer is A. Recombinant vectors maintain करना / Maintaining recombinant vectors. In both libraries DNA fragments are cloned into vectors. The competent host maintains these vectors.
Step 3
Exam Tip
दोनों libraries में DNA fragments vectors में clone होते हैं। Competent host इन vectors को maintain करता है।
A. Host vector insert और marker की safety समझने के लिए/To understand safety of host vector insert and marker
Step 1
Concept
Risk assessment decides safe handling and containment. Host vector insert and marker are considered.
Step 2
Why this answer is correct
The correct answer is A. Host vector insert और marker की safety समझने के लिए / To understand safety of host vector insert and marker. Risk assessment decides safe handling and containment. Host vector insert and marker are considered.
Step 3
Exam Tip
Risk assessment safe handling और containment तय करता है। इसमें host vector insert और marker consider होते हैं।
Non-pathogenic strains reduce biosafety risk. Therefore they are preferred in educational and routine lab work.
Step 2
Why this answer is correct
The correct answer is A. Safer handling के लिए / For safer handling. Non-pathogenic strains reduce biosafety risk. Therefore they are preferred in educational and routine lab work.
Step 3
Exam Tip
Non-pathogenic strains biosafety risk कम करते हैं। इसलिए educational और routine lab work में इन्हें prefer किया जाता है।
A. Outside environment में survival कम हो सकता है/Survival outside environment may decrease
Step 1
Concept
Auxotrophic strains can depend on special nutrients. This can reduce uncontrolled survival.
Step 2
Why this answer is correct
The correct answer is A. Outside environment में survival कम हो सकता है / Survival outside environment may decrease. Auxotrophic strains can depend on special nutrients. This can reduce uncontrolled survival.
Step 3
Exam Tip
Auxotrophic strains special nutrients पर depend कर सकते हैं। इससे uncontrolled survival कम हो सकती है।
A. Resistance gene spread रोकना जरूरी है/Spread of resistance gene must be prevented
Step 1
Concept
Resistance markers should be handled with containment. Waste disposal should also be safe.
Step 2
Why this answer is correct
The correct answer is A. Resistance gene spread रोकना जरूरी है / Spread of resistance gene must be prevented. Resistance markers should be handled with containment. Waste disposal should also be safe.
Step 3
Exam Tip
Resistance markers को containment के साथ handle करना चाहिए। Waste disposal भी safe होना चाहिए।
A. Accidental spread और contamination रोकने के लिए/To prevent accidental spread and contamination
Step 1
Concept
A spill may contain live cells. Biosafety protocol helps prevent accidental spread.
Step 2
Why this answer is correct
The correct answer is A. Accidental spread और contamination रोकने के लिए / To prevent accidental spread and contamination. A spill may contain live cells. Biosafety protocol helps prevent accidental spread.
Step 3
Exam Tip
Spill में live cells हो सकती हैं। Biosafety protocol accidental spread को रोकने में मदद करता है।
A. Live microbes inactivate करने के लिए/To inactivate live microbes
Step 1
Concept
Autoclaving can inactivate microbes using heat and pressure. It is a common method for safe disposal.
Step 2
Why this answer is correct
The correct answer is A. Live microbes inactivate करने के लिए / To inactivate live microbes. Autoclaving can inactivate microbes using heat and pressure. It is a common method for safe disposal.
Step 3
Exam Tip
Autoclaving heat और pressure से microbes को inactivate कर सकता है। यह safe disposal का common method है।
A. Traceability बनाए रखने के लिए/To maintain traceability
Step 1
Concept
Records prevent wrong sample mix-ups. Strain and plasmid information helps reproducibility.
Step 2
Why this answer is correct
The correct answer is A. Traceability बनाए रखने के लिए / To maintain traceability. Records prevent wrong sample mix-ups. Strain and plasmid information helps reproducibility.
Step 3
Exam Tip
Records गलत sample mix-up रोकते हैं। Strain और plasmid information reproducibility में मदद करती है।
A. Correct conclusions और safety decisions के लिए/For correct conclusions and safety decisions
Step 1
Concept
False results can lead to wrong conclusions and unsafe decisions. Honest reporting is part of responsible biotechnology.
Step 2
Why this answer is correct
The correct answer is A. Correct conclusions और safety decisions के लिए / For correct conclusions and safety decisions. False results can lead to wrong conclusions and unsafe decisions. Honest reporting is part of responsible biotechnology.
Step 3
Exam Tip
False results wrong conclusions और unsafe decisions दे सकते हैं। Honest reporting responsible biotechnology का भाग है।
A. Safety और reproducibility बढ़ती है/Safety and reproducibility increase
Step 1
Concept
An approved protocol reduces mistakes and safety risks. This can make results comparable.
Step 2
Why this answer is correct
The correct answer is A. Safety और reproducibility बढ़ती है / Safety and reproducibility increase. An approved protocol reduces mistakes and safety risks. This can make results comparable.
Step 3
Exam Tip
Approved protocol mistakes और safety risks कम करता है। इससे results comparable हो सकते हैं।
A. Good competent host work में DNA quality controls screening और biosafety साथ चलते हैं/Good competent host work combines DNA quality controls screening and biosafety
Step 1
Concept
Transformation success is not only growth. Controls screening confirmation and safety are also necessary.
Step 2
Why this answer is correct
The correct answer is A. Good competent host work में DNA quality controls screening और biosafety साथ चलते हैं / Good competent host work combines DNA quality controls screening and biosafety. Transformation success is not only growth. Controls screening confirmation and safety are also necessary.
Step 3
Exam Tip
Transformation success केवल growth नहीं है। Controls screening confirmation और safety भी जरूरी हैं।