Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 251-260 of 290 questions.
Which enzyme has its source linked with T4 bacteriophage?
B. T4 DNA ligase
T4 DNA ligase T4 bacteriophage से संबंधित है। यह cloning में commonly used joining enzyme है। / T4 DNA ligase is related to T4 bacteriophage. It is a commonly used joining enzyme in cloning.
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Which type of bond does DNA ligase repair or create?
C. phosphodiester bond in DNA
DNA ligase DNA backbone में phosphodiester bond repair या create करता है। यह DNA continuity के लिए जरूरी है। / DNA ligase repairs or creates phosphodiester bonds in the DNA backbone. It is needed for DNA continuity.
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Which option correctly relates ligase and Okazaki fragments?
D. Ligase Okazaki fragments जोड़ता हैLigase joins Okazaki fragments
Ligase Okazaki fragments जोड़ता है और lagging strand को continuous बनाता है। यह replication का important point है। / Ligase joins Okazaki fragments and makes the lagging strand continuous. This is an important point in replication.
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What happens on the lagging strand in absence of DNA ligase?
A. Okazaki fragments जुड़े नहीं रहेंगेOkazaki fragments will not remain joined
Ligase absent होने पर Okazaki fragments के बीच nicks seal नहीं होंगे। इससे lagging strand incomplete रहेगा। / If ligase is absent nicks between Okazaki fragments will not be sealed. The lagging strand will remain incomplete.
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Ligase forms a covalent bond between what?
A. 3 prime OH और 5 prime phosphate3 prime OH and 5 prime phosphate
DNA ligase 3 prime OH और 5 prime phosphate के बीच covalent phosphodiester bond बनाता है। यही ligation का chemical basis है। / DNA ligase forms a covalent phosphodiester bond between 3 prime OH and 5 prime phosphate. This is the chemical basis of ligation.
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What can be the effect of removing 5 prime phosphate in a ligase reaction?
A. self-ligation घट सकती हैSelf-ligation can decrease
Vector से 5 prime phosphate हटाने पर उसके अपने ends की joining घट सकती है। यह self-ligation control में मदद करता है। / Removing 5 prime phosphate from vector can reduce joining of its own ends. This helps control self-ligation.
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Which treatment can reduce vector self-ligation?
A. alkaline phosphatase treatment
Alkaline phosphatase vector ends से phosphate हटा सकता है। इससे vector self-ligation कम हो सकती है। / Alkaline phosphatase can remove phosphate from vector ends. This can reduce vector self-ligation.
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If vector is dephosphorylated what may be important for insert ligation?
A. insert के पास सही phosphate ends होंInsert has proper phosphate ends
Dephosphorylated vector self-ligation घटाता है लेकिन proper insert ends ligation में मदद करते हैं। Chemical ends का ध्यान जरूरी है। / Dephosphorylated vector reduces self-ligation but proper insert ends help ligation. Chemical ends must be considered.
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In directional cloning what does ligase join?
A. compatible designed ends of insert and vector
Directional cloning में अलग ends design किए जाते हैं। Ligase compatible insert और vector ends को जोड़ता है। / In directional cloning different ends are designed. Ligase joins compatible insert and vector ends.
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What is the benefit of directional cloning in ligation?
A. insert orientation control हो सकता हैInsert orientation can be controlled
Directional cloning insert को desired orientation में जोड़ने में मदद करती है। Ligase फिर सही ends को स्थायी रूप से जोड़ता है। / Directional cloning helps join the insert in the desired orientation. Ligase then permanently joins the correct ends.
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