Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
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Showing 71-80 of 294 questions.
What is the benefit of DNA isolation from a fresh sample?
B. DNA quality बेहतर हो सकती हैDNA quality can be better
Fresh samples में DNA degradation कम हो सकती है। Proper storage भी quality बचाता है। / Fresh samples may have less DNA degradation. Proper storage also preserves quality.
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What problem can occur with an old or poorly stored sample?
A. DNA quality घट सकती हैDNA quality may decrease
Poor storage nucleases और degradation को बढ़ा सकती है। इससे yield और integrity प्रभावित होती है। / Poor storage can increase nucleases and degradation. This affects yield and integrity.
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Why is sample homogenization done in DNA isolation?
A. sample को समान रूप से तोड़ने के लिएTo break the sample uniformly
Homogenization tissues को छोटे भागों में तोड़ता है। इससे lysis buffer अच्छी तरह काम करता है। / Homogenization breaks tissues into smaller parts. This allows lysis buffer to work well.
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Why can liquid nitrogen be useful in plant DNA isolation?
A. tissue को जल्दी freeze करके grind करने मेंTo quickly freeze tissue for grinding
Liquid nitrogen tissue को brittle बनाता है और grinding आसान करता है। इससे DNA degradation भी कम हो सकती है। / Liquid nitrogen makes tissue brittle and easier to grind. It can also reduce DNA degradation.
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Why do polyphenols affect DNA isolation in plants?
A. वे DNA के साथ co-purify होकर reactions रोक सकते हैंThey can co-purify with DNA and inhibit reactions
Polyphenols impurities की तरह DNA sample में आ सकते हैं। Plant DNA methods इन्हें हटाने की कोशिश करते हैं। / Polyphenols can enter the DNA sample as impurities. Plant DNA methods try to remove them.
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Why can polysaccharides be a problem in plant DNA isolation?
A. वे solution को viscous बनाकर enzyme reactions में बाधा डाल सकते हैंThey can make solution viscous and inhibit enzyme reactions
Polysaccharides DNA के साथ co-precipitate हो सकते हैं। CTAB method इन्हें घटाने में मदद करता है। / Polysaccharides can co-precipitate with DNA. The CTAB method helps reduce them.
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Why is CTAB buffer useful in plants?
A. polysaccharides हटाने में मदद के लिएTo help remove polysaccharides
CTAB plant samples में polysaccharides और कुछ impurities को हटाने में मदद करता है। यह plant DNA isolation में common है। / CTAB helps remove polysaccharides and some impurities in plant samples. It is common in plant DNA isolation.
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What can be the role of chloroform in DNA isolation?
A. phase separation और protein removal में मददHelping phase separation and protein removal
Chloroform organic extraction में phases अलग करने में मदद करता है। इससे proteins अलग हो सकते हैं। / Chloroform helps separate phases in organic extraction. This can separate proteins.
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Why is caution necessary while using phenol?
A. क्योंकि यह hazardous chemical हैBecause it is a hazardous chemical
Phenol corrosive और hazardous हो सकता है। इसलिए safety guidelines के अनुसार ही प्रयोग करें। / Phenol can be corrosive and hazardous. Therefore use it only according to safety guidelines.
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Why is there a silica column wash step in DNA isolation?
A. bound DNA से salts और impurities हटाने के लिएTo remove salts and impurities from bound DNA
Wash buffer DNA को column पर रखते हुए impurities हटाता है। फिर elution में DNA recover होता है। / Wash buffer removes impurities while DNA remains on the column. DNA is then recovered in elution.
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