Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
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Showing 61-70 of 294 questions.
What problem occurs when a DNA pellet is over-dried?
D. DNA dissolve करना कठिन हो जाता हैDNA becomes difficult to dissolve
बहुत अधिक सूखा pellet buffer में जल्दी नहीं घुलता। सही drying time जरूरी है। / An over-dried pellet does not dissolve quickly in buffer. Proper drying time is important.
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Why is protein contamination a problem in a DNA sample?
A. यह downstream reactions में बाधा डाल सकती हैIt can interfere with downstream reactions
Protein contamination enzyme reactions और purity readings को प्रभावित कर सकती है। Protein removal जरूरी है। / Protein contamination can affect enzyme reactions and purity readings. Protein removal is necessary.
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What can a very low A260 by A280 ratio indicate?
B. Protein contamination
कम A260 by A280 ratio protein या phenol contamination का संकेत दे सकता है। Ratio को quality check में याद रखें। / A low A260 by A280 ratio can indicate protein or phenol contamination. Remember the ratio in quality check.
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A260 by A230 ratio can indicate contamination by what?
C. salt phenol या carbohydratesSalt phenol or carbohydrates
A260 by A230 ratio salts phenol और carbohydrates जैसी impurities का संकेत दे सकता है। यह purity assessment में उपयोगी है। / The A260 by A230 ratio can indicate impurities like salts phenol and carbohydrates. It is useful in purity assessment.
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Why is absorbance reading useful in DNA quantification?
D. DNA concentration estimate करने के लिएTo estimate DNA concentration
DNA 260 nm पर absorb करता है और reading concentration estimate कर सकती है। यह sample normalization में मदद करता है। / DNA absorbs at 260 nm and the reading can estimate concentration. It helps in sample normalization.
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How can intact genomic DNA appear on agarose gel?
A. उच्च molecular weight band के रूप मेंAs a high molecular weight band
Intact genomic DNA बड़े size का band दे सकता है। Degraded DNA smear दिखा सकता है। / Intact genomic DNA can give a large size band. Degraded DNA can show a smear.
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Due to which charge does DNA move in gel electrophoresis?
B. नकारात्मक chargeNegative charge
DNA phosphate backbone के कारण negatively charged होता है। इसलिए electric field में positive electrode की ओर move करता है। / DNA is negatively charged due to its phosphate backbone. Therefore it moves toward the positive electrode in an electric field.
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Which property of the phosphate backbone is useful in DNA isolation?
C. negative charge
DNA का negative charge precipitation और gel movement जैसे steps में महत्वपूर्ण है। Salt charge shielding में मदद करता है। / The negative charge of DNA is important in steps like precipitation and gel movement. Salt helps in charge shielding.
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Why is it important to protect DNA from excessive mechanical force?
D. DNA shearing रोकने के लिएTo prevent DNA shearing
Mechanical force लंबे DNA को छोटे fragments में तोड़ सकता है। इसलिए gentle pipetting बेहतर है। / Mechanical force can break long DNA into smaller fragments. Therefore gentle pipetting is better.
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Why is gentle pipetting done in DNA extraction?
A. DNA टूटने से बचाने के लिएTo prevent DNA breakage
Gentle pipetting genomic DNA को shear होने से बचाती है। यह high molecular weight DNA के लिए जरूरी है। / Gentle pipetting protects genomic DNA from shearing. It is important for high molecular weight DNA.
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