अगर vector ends dephosphorylated हैं तो self-ligation क्यों घट सकती है?
Why can self-ligation decrease if vector ends are dephosphorylated?
Explanation opens after your attempt
A. 5 prime phosphate हटने से vector अपने ends आसानी से नहीं जोड़ पाएगाRemoval of 5 prime phosphate makes vector ends less able to join themselves
Concept
Removing 5 prime phosphate can reduce vector self-ligation. This is a strategy to reduce unwanted background in cloning.
Why this answer is correct
The correct answer is A. 5 prime phosphate हटने से vector अपने ends आसानी से नहीं जोड़ पाएगा / Removal of 5 prime phosphate makes vector ends less able to join themselves. Removing 5 prime phosphate can reduce vector self-ligation. This is a strategy to reduce unwanted background in cloning.
Exam Tip
5 prime phosphate हटने पर vector self-ligation कम हो सकती है। यह cloning में unwanted background घटाने की strategy है।
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