Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 211-220 of 295 questions.
Cos sites in a cosmid vector come from what?
A. Lambda phage
Cosmid में lambda phage के cos sites शामिल होते हैं। यह packaging से जुड़ा vector feature है। / A cosmid includes cos sites from lambda phage. This is a vector feature linked with packaging.
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What biological component is the basis of a phage vector?
A. Bacteriophage
Bacteriophage bacteria को infect करने वाला virus है। Modified phages cloning vectors के रूप में उपयोग हो सकते हैं। / A bacteriophage is a virus that infects bacteria. Modified phages can be used as cloning vectors.
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Which vector can be considered different from plasmid due to large insert capacity?
A. BAC
BAC large inserts को carry करने में useful है। Simple plasmids आमतौर पर smaller inserts के लिए सुविधाजनक होते हैं। / BAC is useful for carrying large inserts. Simple plasmids are usually convenient for smaller inserts.
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A host cell does not take the vector. What will happen on an antibiotic plate?
A. Resistance marker न होने से growth नहीं होगीIt will not grow because it lacks resistance marker
Non-transformed cell में resistance marker नहीं होता। इसलिए antibiotic plate पर वह बढ़ नहीं सकती। / A non-transformed cell lacks the resistance marker. Therefore it cannot grow on an antibiotic plate.
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What is the purpose of making competent cells?
A. DNA uptake आसान करनाMaking DNA uptake easier
Competent cells external DNA लेने के लिए तैयार होती हैं। Transformation में यह important step है। / Competent cells are prepared to take up external DNA. This is an important step in transformation.
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What is the purpose of heat shock transformation?
A. Bacterial cells में plasmid entry में मददHelping plasmid enter bacterial cells
Heat shock competent bacteria में plasmid uptake बढ़ाने के लिए प्रयोग किया जा सकता है। यह transformation method का हिस्सा है। / Heat shock can be used to increase plasmid uptake in competent bacteria. It is part of a transformation method.
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In electroporation vector uptake is increased by what?
A. Electric pulse
Electroporation में electric pulse से cell membrane temporarily permeable हो सकती है। इससे DNA uptake बढ़ सकता है। / In electroporation an electric pulse can make the cell membrane temporarily permeable. This can increase DNA uptake.
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Why is a gel band checked for insert confirmation in a cloning vector?
A. Expected size का DNA fragment देखने के लिएTo see a DNA fragment of expected size
Expected band insert की presence और size का संकेत दे सकता है। यह restriction analysis के बाद देखा जाता है। / An expected band can indicate insert presence and size. It is checked after restriction analysis.
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What can be confirmed in a cloning vector by sequencing?
A. Insert sequence और orientationInsert sequence and orientation
Sequencing insert का सही sequence और direction confirm कर सकती है। यह final confirmation में उपयोगी है। / Sequencing can confirm the correct sequence and direction of the insert. It is useful for final confirmation.
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Why can an empty vector colony survive selection?
A. उसमें marker हो सकता है लेकिन insert नहींIt may have marker but no insert
Empty vector marker के कारण antibiotic पर बढ़ सकता है। लेकिन desired insert न होने से screening जरूरी है। / An empty vector can grow on antibiotic due to the marker. But screening is needed because it lacks the desired insert.
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