Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 201-210 of 295 questions.
In blue-white selection what does a blue colony generally indicate?
A. Non-recombinant vector
Blue colony lacZ active रहने का संकेत हो सकती है। इसका अर्थ सामान्यतः insert न होना है। / A blue colony can indicate active lacZ. It generally means the insert is absent.
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A colony grew on an antibiotic plate but insert is not confirmed. What is needed?
A. Further screening या confirmationFurther screening or confirmation
Antibiotic growth vector uptake दिखाता है। Insert की पुष्टि के लिए screening या analysis जरूरी हो सकता है। / Antibiotic growth shows vector uptake. Screening or analysis may be needed to confirm the insert.
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Which option correctly distinguishes selection and screening?
A. Selection transformed cells चुनता है और screening recombinants पहचानती हैSelection chooses transformed cells and screening identifies recombinants
Selection vector लेने वाली cells चुनती है। Screening insert वाले recombinants पहचानती है। / Selection chooses cells that received the vector. Screening identifies recombinants carrying the insert.
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What is the use of ampR in pBR322?
A. Ampicillin resistance देनाGiving ampicillin resistance
ampR ampicillin resistance देता है। इससे transformed cells को ampicillin medium पर select किया जा सकता है। / ampR gives ampicillin resistance. It allows selection of transformed cells on ampicillin medium.
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In pBR322 tetR is an example of what?
A. Antibiotic resistance marker
tetR tetracycline resistance से जुड़ा selectable marker है। इसे pBR322 के marker genes में याद रखें। / tetR is a selectable marker linked with tetracycline resistance. Remember it among pBR322 marker genes.
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Why are pUC vectors useful due to high copy number?
A. अधिक plasmid DNA मिल सकता हैMore plasmid DNA can be obtained
High copy number से प्रति cell plasmid copies बढ़ती हैं। इससे plasmid DNA yield अधिक हो सकती है। / High copy number increases plasmid copies per cell. This can give higher plasmid DNA yield.
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In which vector is lacZ-based blue-white screening commonly studied?
A. pUC vectors
pUC vectors lacZ आधारित blue-white screening से जुड़े होते हैं। यह recombinant screening का सरल example है। / pUC vectors are linked with lacZ-based blue-white screening. This is a simple example of recombinant screening.
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T-DNA in Ti plasmid is linked with what?
A. Plant cell में DNA transferDNA transfer into plant cell
T-DNA पौधे की कोशिका में transfer हो सकता है। modified Ti plasmid plant biotechnology में उपयोगी है। / T-DNA can be transferred into plant cells. Modified Ti plasmid is useful in plant biotechnology.
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What is the major use of BAC vector?
A. Large DNA insert cloning
BAC large DNA fragments cloning के लिए useful है। इसे bacterial artificial chromosome के रूप में याद रखें। / BAC is useful for cloning large DNA fragments. Remember it as bacterial artificial chromosome.
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YAC vector can be useful for which type of inserts?
A. बहुत बड़े डीएनए insertsVery large DNA inserts
YAC very large DNA inserts carry करने में उपयोगी हो सकता है। यह yeast artificial chromosome है। / YAC can be useful for carrying very large DNA inserts. It is yeast artificial chromosome.
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