Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 181-190 of 295 questions.
What is the empty vector problem?
A. Vector में desired insert नहीं होताVector lacks desired insert
Empty vector selection से बच सकता है लेकिन desired gene नहीं रखता। इसलिए screening जरूरी होती है। / An empty vector may survive selection but lacks the desired gene. Therefore screening is needed.
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Why is host compatibility important in a cloning vector?
A. Ori host की replication machinery के साथ काम करेOri should work with host replication machinery
Vector का ori host में काम करना चाहिए। तभी vector replicate होकर copies बना सकेगा। / The vector ori should work in the host. Only then can the vector replicate and make copies.
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What is the correct meaning of a competent cell?
A. बाहरी डीएनए लेने के लिए तैयार कोशिकाCell prepared to take up external DNA
Competent cells बाहरी DNA uptake के लिए तैयार की जाती हैं। Transformation में यह step उपयोगी है। / Competent cells are prepared for external DNA uptake. This step is useful in transformation.
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Why can insert orientation be important in a cloning vector?
A. Expression या आगे के प्रयोग प्रभावित हो सकते हैंExpression or downstream experiments can be affected
Insert की दिशा gene expression या analysis को प्रभावित कर सकती है। Directional cloning orientation control में मदद करती है। / Insert direction can affect gene expression or analysis. Directional cloning helps control orientation.
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Why can two different restriction enzymes be used in directional cloning?
A. Insert को सही दिशा में जोड़ने के लिएTo join insert in correct orientation
दो अलग enzymes अलग ends बनाते हैं। इससे insert की direction control करना आसान होता है। / Two different enzymes make different ends. This makes it easier to control insert direction.
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Which combination is most correct for making a recombinant vector?
A. Vector plus insert DNA plus DNA ligase
Recombinant vector बनाने के लिए insert को vector से जोड़ा जाता है। DNA ligase इस joining में मदद करता है। / To make a recombinant vector the insert is joined with the vector. DNA ligase helps in this joining.
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Which marker allows transformed bacteria to grow on antibiotic?
A. Antibiotic resistance gene
Antibiotic resistance gene bacteria को संबंधित antibiotic पर बढ़ने देता है। इससे transformed cells select होती हैं। / An antibiotic resistance gene allows bacteria to grow on the related antibiotic. This selects transformed cells.
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Which statement about a cloning vector is incorrect?
D. यह हमेशा starch digestion enzyme होता हैIt is always a starch-digesting enzyme
Cloning vector DNA carrier होता है enzyme नहीं। इसका काम foreign DNA को host में ले जाना है। / A cloning vector is a DNA carrier not a digestive enzyme. Its role is to carry foreign DNA into a host.
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Which task becomes difficult in gene cloning without a cloning vector?
A. Desired DNA को host में carry करनाCarrying desired DNA into host
Vector desired DNA को host में carry करता है। इसके बिना desired DNA copies बनवाना कठिन होगा। / A vector carries desired DNA into a host. Without it making copies of desired DNA becomes difficult.
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Which part can affect vector copy number?
A. ओरीOri
Ori replication control से जुड़ा होता है। इसलिए यह vector copy number को प्रभावित कर सकता है। / Ori is linked with replication control. Therefore it can affect vector copy number.
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