Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 31-40 of 294 questions.
DNA absorbance is generally measured at which wavelength?
C. 260 nm
DNA ultraviolet light को लगभग 260 nm पर absorb करता है। इसलिए DNA quantification में 260 nm important है। / DNA absorbs ultraviolet light around 260 nm. Therefore 260 nm is important for DNA quantification.
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Which ratio can indicate protein contamination?
D. A260 by A280
A260 by A280 ratio DNA purity का संकेत देता है। कम value protein contamination की ओर इशारा कर सकती है। / The A260 by A280 ratio indicates DNA purity. A low value can suggest protein contamination.
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What approximate A260 by A280 ratio is considered good for pure DNA?
A. लगभग 1.8About 1.8
Pure DNA का A260 by A280 ratio लगभग 1.8 माना जाता है। यह purity का exam-friendly value है। / Pure DNA is considered to have an A260 by A280 ratio of about 1.8. This is an exam-friendly value.
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Why is agarose gel electrophoresis done after DNA isolation?
A. डीएनए की presence और integrity देखने के लिएTo check DNA presence and integrity
Agarose gel पर DNA bands देखे जा सकते हैं। Smearing DNA degradation का संकेत दे सकती है। / DNA bands can be seen on an agarose gel. Smearing can indicate DNA degradation.
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What can smearing on a gel indicate?
A. डीएनए degradationDNA degradation
Smear टूटे हुए DNA fragments का संकेत हो सकता है। Gentle handling और nuclease protection जरूरी हैं। / A smear can indicate broken DNA fragments. Gentle handling and nuclease protection are necessary.
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Why is wearing gloves important in DNA isolation?
A. Contamination और nuclease exposure कम करने के लिएTo reduce contamination and nuclease exposure
हाथों से nucleases और contaminants आ सकते हैं। Gloves sample protection में मदद करते हैं। / Hands can introduce nucleases and contaminants. Gloves help protect samples.
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Why are sterile nuclease-free tubes useful in DNA isolation?
A. डीएनए degradation और contamination घटाने के लिएTo reduce DNA degradation and contamination
Nuclease-free tubes DNA को टूटने से बचाने में मदद करती हैं। Sterility contamination को कम करती है। / Nuclease-free tubes help protect DNA from breakdown. Sterility reduces contamination.
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What can leftover ethanol in a DNA sample do to later experiments?
B. PCR या enzyme reactions को inhibit कर सकता हैCan inhibit PCR or enzyme reactions
Residual ethanol downstream reactions को रोक सकता है। DNA pellet को सही तरह सुखाना चाहिए। / Residual ethanol can inhibit downstream reactions. The DNA pellet should be dried properly.
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What problem can occur if a DNA pellet is over-dried?
A. DNA dissolve करना कठिन हो सकता हैDNA may become difficult to dissolve
बहुत dry pellet buffer में धीरे dissolve हो सकता है। उचित drying important है। / An over-dried pellet may dissolve slowly in buffer. Proper drying is important.
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What is a main difference between genomic DNA and plasmid DNA isolation?
A. Genomic DNA chromosome से और plasmid DNA extra-chromosomal plasmid से मिलता हैGenomic DNA comes from chromosome and plasmid DNA from extra-chromosomal plasmid
Genomic DNA पूरा chromosomal DNA होता है जबकि plasmid DNA छोटा circular extra-chromosomal DNA होता है। Method का goal अलग हो सकता है। / Genomic DNA is whole chromosomal DNA while plasmid DNA is small circular extra-chromosomal DNA. The method goal can differ.
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