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Subjects

Biotechnology

Insertion

In this Class 11 Biotechnology topic, students learn how a desired DNA fragment is inserted into a suitable cloning vector during recombinant DNA technology. The topic explains the role of restriction enzymes in creating compatible ends, selection of an appropriate insertion site, and DNA ligase in joining the fragment with the vector to form recombinant DNA. It also introduces how insertion can affect a marker gene and why transformed cells are later identified through selection and screening.

Easy · Level 1 · 1 questions

Practice questions

01What is insert DNA?

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Answer and explanation

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