Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
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Showing 241-250 of 292 questions.
Which option is the best revision point for ligase topic?
A. joins DNA by phosphodiester bond
Ligase को DNA joining और phosphodiester bond formation से याद करें। यही topic का core है। / Remember ligase with DNA joining and phosphodiester bond formation. This is the core of the topic.
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In Class 11 in which two contexts should ligase enzymes be remembered?
A. recombinant DNA और DNA replicationRecombinant DNA and DNA replication
Ligase recombinant DNA में fragments जोड़ता है और replication में Okazaki fragments जोड़ता है। ये दोनों exam contexts महत्वपूर्ण हैं। / Ligase joins fragments in recombinant DNA and joins Okazaki fragments in replication. Both exam contexts are important.
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What is a safe one-line answer for ligase enzymes?
A. ये ऊर्जा की मदद से DNA ends जोड़ते हैंThey join DNA ends with the help of energy
Ligase enzymes energy की सहायता से DNA ends को जोड़ते हैं। DNA ligase phosphodiester bond बनाता है। / Ligase enzymes join DNA ends with the help of energy. DNA ligase forms a phosphodiester bond.
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In a practical cut vector and insert are mixed. What should be added for stable joining?
A. डीएनए लाइगेजDNA ligase
डीएनए लाइगेज cut vector और insert के बीच phosphodiester bond बनाता है। इससे stable recombinant DNA बन सकता है। / DNA ligase forms phosphodiester bonds between cut vector and insert. This can make stable recombinant DNA.
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If ligase is not added to a cloning mixture what will be missing?
B. permanent DNA joining
Ligase न होने पर permanent phosphodiester joining नहीं होगी। Sticky ends केवल temporary pair कर सकते हैं। / Without ligase permanent phosphodiester joining will not occur. Sticky ends can only pair temporarily.
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Which enzyme seals nicks between DNA fragments?
C. DNA ligase
DNA ligase DNA fragments के बीच nick seal करता है। इसे DNA replication और cloning दोनों में याद रखें। / DNA ligase seals nicks between DNA fragments. Remember it in both DNA replication and cloning.
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In which step is ligase useful?
D. डीएनए खंडों को जोड़नाJoining DNA fragments
डीएनए लाइगेज डीएनए के दो खंडों के बीच फॉस्फोडाइएस्टर बंध बनाकर उन्हें जोड़ता है; इसलिए पुनर्संयोजक डीएनए बनाने के ligation step में इसका उपयोग होता है। restriction enzyme डीएनए को काटता है, जबकि gel electrophoresis खंडों को अलग करती है। परीक्षा टिप: “ligase” को DNA fragments को “join” करने वाले एंजाइम के रूप में याद रखें। / DNA ligase joins two DNA fragments by forming phosphodiester bonds between them. Therefore, it is used in the ligation step of recombinant DNA formation. A restriction enzyme cuts DNA, whereas gel electrophoresis separates DNA fragments. Exam tip: remember “ligase” as the enzyme that “joins” DNA fragments.
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What makes a recombinant plasmid stable?
A. vector और insert के बीच phosphodiester bondPhosphodiester bond between vector and insert
Ligase vector और insert के बीच phosphodiester bond बनाता है। यही recombinant plasmid को stable बनाता है। / Ligase forms a phosphodiester bond between vector and insert. This makes the recombinant plasmid stable.
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By which process can sticky ends first come together?
B. complementary base pairing
Sticky ends में complementary overhangs होते हैं। वे पहले base pairing से पास आ सकते हैं और फिर ligase उन्हें जोड़ता है। / Sticky ends have complementary overhangs. They can first come together by base pairing and then ligase joins them.
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Why can ligation with blunt ends be difficult?
C. उनमें overhang नहीं होताThey have no overhang
Blunt ends में complementary overhang नहीं होता। इसलिए sticky ends की तरह initial pairing support नहीं मिलता। / Blunt ends do not have complementary overhangs. Therefore they lack initial pairing support like sticky ends.
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