लाइगेज के बिना cloning में क्या मुख्य समस्या होगी?
Without ligase what will be the main problem in cloning?
Explanation opens after your attempt
Correct Answer
D. cut vector और insert स्थायी रूप से नहीं जुड़ेंगेCut vector and insert will not join permanently
Concept
Restriction enzyme can cut but ligase performs permanent joining. Therefore ligase is essential in cloning.
Why this answer is correct
The correct answer is D. cut vector और insert स्थायी रूप से नहीं जुड़ेंगे / Cut vector and insert will not join permanently. Restriction enzyme can cut but ligase performs permanent joining. Therefore ligase is essential in cloning.
Exam Tip
Restriction enzyme cutting कर सकता है लेकिन permanent joining ligase करता है। इसलिए ligase cloning में आवश्यक है।
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