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Biotechnology Competent Host MCQ Questions for Class 11 Science

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01 Practice Topic Genetic Engineering 200 MCQs 02 Practice Topic Bioprocess Engineering 300 MCQs 03 Practice Topic Restriction Enzymes 345 MCQs 04 Practice Topic Ligase Enzymes 290 MCQs 05 Practice Topic Cloning Vectors 295 MCQs

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Question 311/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

किस कारण competent cells की efficiency storage के दौरान घट सकती है?

Why can the efficiency of competent cells decrease during storage?

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A. गलत temperature या repeated thawingWrong temperature or repeated thawing

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Poor storage और repeated freeze-thaw cells को नुकसान पहुँचा सकते हैं। Aliquots efficiency बचाने में मदद करते हैं। / Poor storage and repeated freeze-thaw can damage cells. Aliquots help preserve efficiency.

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Question 312/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Transformation में gentle mixing क्यों बेहतर है?

Why is gentle mixing better in transformation?

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A. Competent cells को damage से बचाने के लिएTo protect competent cells from damage

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Competent cells fragile हो सकती हैं। Gentle handling viability और competence को बचाता है। / Competent cells can be fragile. Gentle handling preserves viability and competence.

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Question 313/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Electroporation में high salt sample क्यों avoid किया जाता है?

Why is a high salt sample avoided in electroporation?

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A. Arcing और cell damage रोकने के लिएTo prevent arcing and cell damage

Explanation

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High salt electric pulse में arcing कर सकता है। Clean low-salt DNA electroporation में बेहतर होता है। / High salt can cause arcing during electric pulse. Clean low-salt DNA is better for electroporation.

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Question 314/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

रासायनिक competence में divalent cations का role क्या है?

What is the role of divalent cations in chemical competence?

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A. DNA और cell surface के charge barrier को घटानाReducing charge barrier between DNA and cell surface

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Divalent cations DNA uptake को आसान बना सकते हैं। Calcium ions इसका common example हैं। / Divalent cations can make DNA uptake easier. Calcium ions are a common example.

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Question 315/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

किस condition में recombinant clone false positive हो सकता है?

In which condition can a recombinant clone be false positive?

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A. Empty vector marker के कारण select हो जाएEmpty vector is selected because of marker

Explanation

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Empty vector marker के कारण selection plate पर grow कर सकता है। Screening false positives हटाने में मदद करती है। / An empty vector can grow on a selection plate due to the marker. Screening helps remove false positives.

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Question 316/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Blue-white screening में white colony अक्सर किसका संकेत हो सकती है?

In blue-white screening what can a white colony often indicate?

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A. Insert disruption वाला recombinant cloneRecombinant clone with insert disruption

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lacZ disruption white colony का संकेत दे सकता है। फिर भी final confirmation molecular method से करें। / lacZ disruption can indicate a white colony. Still final confirmation should be done by molecular methods.

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Question 317/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Blue-white screening में blue colony सामान्यतः क्या बता सकती है?

In blue-white screening what can a blue colony generally indicate?

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A. Non-recombinant vector

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Blue colony अक्सर intact lacZ के कारण non-recombinant vector दिखाती है। Screening result को confirmation से verify करें। / A blue colony often indicates non-recombinant vector due to intact lacZ. Verify screening results by confirmation.

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Question 318/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Competent host में colony PCR किस चीज की quick जाँच कर सकता है?

What can colony PCR quickly check in a competent host?

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A. Target insert की उपस्थितिPresence of target insert

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Colony PCR selected colony में insert presence का quick test है। Final sequence check के लिए sequencing बेहतर है। / Colony PCR is a quick test for insert presence in a selected colony. Sequencing is better for final sequence check.

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Question 319/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Restriction analysis competent host work में क्यों किया जाता है?

Why is restriction analysis done in competent host work?

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A. Plasmid pattern और insert size check करने के लिएTo check plasmid pattern and insert size

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Restriction digest expected band pattern दे सकता है। यह clone confirmation में उपयोगी है। / Restriction digest can give an expected band pattern. It is useful in clone confirmation.

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Question 320/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 36

Sequencing competent host से मिले clone में क्या confirm कर सकती है?

What can sequencing confirm in a clone obtained from a competent host?

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A. Insert की exact sequenceExact sequence of insert

Explanation

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Sequencing exact sequence और mutation status बता सकती है। यह final confirmation के लिए मजबूत method है। / Sequencing can show exact sequence and mutation status. It is a strong method for final confirmation.

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