Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 321-330 of 445 questions.
What can increase plasmid stability in a competent host?
A. उचित selection pressureProper selection pressure
Selection pressure plasmid-containing cells को बनाए रखता है। इससे plasmid loss कम हो सकता है। / Selection pressure maintains plasmid-containing cells. This can reduce plasmid loss.
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What can happen in a transformed culture if selection pressure is absent?
A. Plasmid loss हो सकता हैPlasmid loss can occur
Selection न हो तो कुछ cells plasmid खो सकती हैं। Maintenance के लिए suitable selection उपयोगी है। / Without selection some cells can lose the plasmid. Suitable selection is useful for maintenance.
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What is a possible drawback of a high copy plasmid in a competent host?
A. Metabolic burden बढ़ सकता हैMetabolic burden can increase
High copy plasmid host resources पर extra load डाल सकता है। Toxic inserts में यह problem बढ़ सकती है। / A high copy plasmid can put extra load on host resources. This problem can increase with toxic inserts.
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When can a low copy plasmid be better in a competent host?
A. Toxic या unstable insert के लिएFor toxic or unstable insert
Low copy vector host burden कम कर सकता है। यह toxic या unstable inserts को maintain करने में मदद कर सकता है। / A low copy vector can reduce host burden. It can help maintain toxic or unstable inserts.
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Which type of host can be useful for a recombination-prone insert?
A. Recombination-deficient host
Recombination-deficient host rearrangement risk घटा सकता है। Repeated sequences वाले inserts में यह उपयोगी है। / A recombination-deficient host can reduce rearrangement risk. It is useful for inserts with repeated sequences.
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What benefit can low endonuclease activity give in plasmid preparation?
A. Plasmid DNA degradation कम हो सकती हैPlasmid DNA degradation can decrease
Endonuclease-deficient host plasmid quality बेहतर रख सकता है। यह cloning strain का useful feature है। / An endonuclease-deficient host can keep plasmid quality better. This is a useful feature of cloning strains.
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Why is low protease activity beneficial in an expression host?
A. Recombinant protein breakdown कम होगाRecombinant protein breakdown will decrease
Proteases expressed protein को degrade कर सकते हैं। Protease-deficient host protein stability बढ़ा सकता है। / Proteases can degrade expressed protein. A protease-deficient host can increase protein stability.
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What can be a main reason for inclusion body formation during protein expression?
A. Protein misfolding और aggregationProtein misfolding and aggregation
Misfolded proteins insoluble aggregates बना सकते हैं। Lower temperature और chaperones मदद कर सकते हैं। / Misfolded proteins can form insoluble aggregates. Lower temperature and chaperones can help.
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Why are chaperone proteins useful in an expression host?
A. Protein folding में सहायता के लिएTo help protein folding
Chaperones protein folding में मदद करते हैं। इससे soluble protein yield बेहतर हो सकती है। / Chaperones help protein folding. This can improve soluble protein yield.
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Lower expression temperature can improve which result?
A. Protein solubility
कम temperature कुछ proteins को properly fold होने में मदद कर सकता है। इससे soluble fraction बढ़ सकती है। / Lower temperature can help some proteins fold properly. This can increase the soluble fraction.
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