Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 171-180 of 445 questions.
What does plasmid burden mean?
A. Plasmid maintain करने से host पर extra loadExtra load on host due to plasmid maintenance
Plasmid replication और gene expression host resources use करते हैं। इससे growth slow हो सकती है। / Plasmid replication and gene expression use host resources. This can slow growth.
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Why can a high-copy plasmid cause a problem with a toxic insert?
A. Toxic product अधिक बन सकता हैMore toxic product can be made
High-copy plasmid से toxic gene dosage बढ़ सकता है। Low-copy vector कभी बेहतर विकल्प हो सकता है। / A high-copy plasmid can increase toxic gene dosage. A low-copy vector can sometimes be a better option.
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What does co-transformation mean?
A. एक ही host में दो genetic elements डालनाIntroducing two genetic elements into the same host
Co-transformation में एक host cell दो DNA molecules ले सकती है। Selection design सावधानी से करनी चाहिए। / In co-transformation one host cell can take two DNA molecules. Selection design should be careful.
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Why are two markers kept in a two-plasmid system?
A. दोनों plasmids select और maintain करने के लिएTo select and maintain both plasmids
दो अलग markers दोनों plasmids की उपस्थिति सुनिश्चित करने में मदद करते हैं। इससे co-transformants चुने जा सकते हैं। / Two different markers help ensure presence of both plasmids. This allows selection of co-transformants.
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When is plasmid compatibility group important?
A. जब दो plasmids साथ रखने होंWhen two plasmids must be maintained together
Same compatibility group के plasmids साथ stable नहीं रह सकते। Multiple plasmid systems में यह जरूरी point है। / Plasmids of the same compatibility group may not remain stable together. This is important in multiple plasmid systems.
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A. कौन से hosts vector को maintain कर सकते हैंWhich hosts can maintain a vector
Host range बताता है कि vector किन hosts में काम कर सकता है। Broad host range vectors अधिक hosts में उपयोगी होते हैं। / Host range tells in which hosts a vector can work. Broad host range vectors are useful in more hosts.
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What is the benefit of a broad host range vector?
A. कई host species में काम कर सकता हैIt can work in many host species
Broad host range vector कई bacterial hosts में replicate हो सकता है। यह versatile cloning में मदद करता है। / A broad host range vector can replicate in many bacterial hosts. It helps in versatile cloning.
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What is a limitation of a narrow host range vector?
A. यह केवल कुछ hosts में काम करता हैIt works only in some hosts
Narrow host range vector हर host में replicate नहीं करता। इसलिए host selection ध्यान से करनी होती है। / A narrow host range vector does not replicate in every host. Therefore host selection must be careful.
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Why should a selectable marker be host-compatible?
A. Marker expression और selection काम करने के लिएFor marker expression and selection to work
Marker host में express होना चाहिए और selection condition से match होना चाहिए। तभी transformants चुने जाते हैं। / The marker should express in the host and match the selection condition. Only then transformants are selected.
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Why should a promoter be host-compatible?
A. Host machinery उसे पहचान सकेHost machinery can recognize it
Promoter को host transcription machinery पहचाननी चाहिए। Otherwise gene expression weak या absent हो सकता है। / The host transcription machinery should recognize the promoter. Otherwise gene expression can be weak or absent.
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