Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 161-170 of 445 questions.
lacZ reporter is linked with which screening?
A. नीला-सफेद screeningBlue-white screening
लैकजेड activity colony color बदल सकती है। Insert disruption white colony का संकेत दे सकता है। / lacZ activity can change colony color. Insert disruption can indicate a white colony.
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What can be observed with GFP reporter?
A. फ्लोरेसेंस signalFluorescence signal
GFP green fluorescence दे सकता है। इसे expression signal के उदाहरण की तरह याद रखें। / GFP can give green fluorescence. Remember it as an example of expression signal.
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Which host feature can protect recombinant protein from breakdown?
A. Protease-deficient background
Protease-deficient host protein degradation कम कर सकता है। Expression experiments में यह उपयोगी होता है। / A protease-deficient host can reduce protein degradation. It is useful in expression experiments.
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What is the main benefit of a recombination-deficient host?
A. Cloned insert की stability बढ़ानाIncreasing stability of cloned insert
Recombination-deficient host unwanted rearrangement घटा सकता है। इससे insert अधिक stable रहता है। / A recombination-deficient host can reduce unwanted rearrangement. This keeps the insert more stable.
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Why can an endonuclease-deficient host be useful?
A. Plasmid DNA degradation घटाने के लिएTo reduce plasmid DNA degradation
Endonuclease activity कम होने पर plasmid DNA बेहतर quality में मिल सकता है। यह plasmid preparation में मदद करता है। / With lower endonuclease activity plasmid DNA can be obtained in better quality. This helps plasmid preparation.
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What will happen if the host has fewer tRNAs for rare codons?
A. Protein expression प्रभावित हो सकता हैProtein expression can be affected
Rare codons translation को धीमा कर सकते हैं। Codon optimization या suitable host मदद कर सकते हैं। / Rare codons can slow translation. Codon optimization or a suitable host can help.
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For what purpose can codon optimization be done?
A. Host में protein expression सुधारने के लिएTo improve protein expression in a host
Codon optimization host-preferred codons का उपयोग कर expression बेहतर कर सकता है। इसे recombinant protein production से जोड़ें। / Codon optimization can improve expression by using host-preferred codons. Link it with recombinant protein production.
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If a recombinant protein is toxic what effect can it have on the host?
A. Growth धीमी या cell death हो सकती हैGrowth may slow or cell death may occur
Toxic protein host health को नुकसान पहुँचा सकता है। Controlled expression system उपयोगी हो सकता है। / A toxic protein can harm host health. A controlled expression system can be useful.
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Why is an inducible promoter used?
A. Gene expression को control करने के लिएTo control gene expression
Inducible promoter जरूरत पड़ने पर expression शुरू कराता है। Toxic genes में यह विशेष रूप से उपयोगी हो सकता है। / An inducible promoter starts expression when needed. It can be especially useful for toxic genes.
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What is the simple meaning of a constitutive promoter?
A. Gene लगातार express हो सकता हैGene can be expressed continuously
Constitutive promoter gene को लगातार express करा सकता है। Toxic gene के लिए सावधानी जरूरी है। / A constitutive promoter can make a gene express continuously. Caution is needed for toxic genes.
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