Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 111-120 of 445 questions.
When can DNA clean-up be useful in electroporation?
D. Salt कम करने के लिएTo reduce salt
Electroporation high salt से arcing हो सकती है। DNA clean-up salt और contaminants कम कर सकता है। / High salt can cause arcing in electroporation. DNA clean-up can reduce salt and contaminants.
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Why can circular plasmid be beneficial in transformation?
A. Circular plasmid stable और replicable हो सकता हैCircular plasmid can be stable and replicable
Plasmid vectors सामान्यतः circular DNA होते हैं और host में replicate कर सकते हैं। इसलिए वे cloning में उपयोगी हैं। / Plasmid vectors are usually circular DNA and can replicate in the host. Therefore they are useful in cloning.
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What is needed for a recombinant plasmid to replicate in host?
B. Host-compatible origin of replication
Origin of replication host machinery के साथ compatible होना चाहिए। तभी plasmid copies बनेंगी। / The origin of replication should be compatible with host machinery. Only then plasmid copies will form.
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What happens if plasmid ori does not work in host?
C. Plasmid maintain नहीं होगाPlasmid will not be maintained
Ori काम न करे तो plasmid replicate नहीं करेगा। इससे transformants stable नहीं रहेंगे। / If ori does not work the plasmid will not replicate. Transformants will not remain stable.
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What is the role of an agar plate in a transformation experiment?
D. Colonies grow कराने और selection करने के लिएTo grow colonies and perform selection
Agar plate transformed cells को colonies में grow करने देती है। Antibiotic होने पर selection भी होती है। / An agar plate lets transformed cells grow as colonies. With antibiotic selection also occurs.
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What is the importance of a colony in cloning?
A. एक transformed cell से बनी population हो सकती हैIt can be a population from one transformed cell
Colony एक clone source हो सकती है। इसलिए individual colonies से plasmid या insert check किया जाता है। / A colony can be a source of a clone. Therefore plasmid or insert is checked from individual colonies.
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B. Specific clone को आगे analyze करने के लिएTo analyze a specific clone further
Individual colony pick करके plasmid isolation PCR या screening की जा सकती है। यह clone confirmation step है। / An individual colony can be picked for plasmid isolation PCR or screening. This is a clone confirmation step.
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C. Insert presence quickly check करने के लिएTo quickly check insert presence
Colony PCR selected colony में insert की presence का quick test है। यह final sequencing से पहले screening कर सकता है। / Colony PCR is a quick test for insert presence in a selected colony. It can screen before final sequencing.
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D. Small culture से plasmid DNA isolate करने के लिएTo isolate plasmid DNA from small culture
Miniprep transformed bacteria से plasmid DNA निकालने की common method है। इसके बाद restriction analysis या sequencing की जा सकती है। / Miniprep is a common method to extract plasmid DNA from transformed bacteria. Restriction analysis or sequencing can follow.
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Why is isolated plasmid digested with restriction enzymes?
A. Insert और vector pattern confirm करने के लिएTo confirm insert and vector pattern
Restriction digestion expected bands दिखाकर correct clone का संकेत दे सकता है। यह plasmid confirmation का common तरीका है। / Restriction digestion can show expected bands and indicate the correct clone. It is a common plasmid confirmation method.
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