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Why is repeated freeze-thaw avoided in DNA storage?

Answer and explanation

Correct answer: To reduce the risk of DNA damage or degradation

Repeated freezing and thawing can subject a DNA sample to physical stress and increase the risk of degradation, which may affect its integrity and suitability for later experiments. Therefore, DNA is preferably stored in small aliquots so that only one portion is thawed when needed. Converting DNA into protein is not a purpose of freeze-thawing. Exam tip: Avoiding repeated freeze-thaw cycles is a standard storage practice for sensitive biomolecules.

Related tags

Dna StorageFreeze ThawDna IntegrityDna DegradationBiotechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyClass 11 Mcq

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