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How can inhibitor problems be reduced in soil DNA extraction?

Answer and explanation

Correct answer: By using special clean-up steps to remove humic substances

Soil often contains substances such as humic acids that can inhibit DNA polymerase and interfere with PCR. Special clean-up or purification steps remove these inhibitors and improve DNA quality. Using unpurified DNA directly in PCR can worsen inhibition. Exam tip: remember humic substances as major PCR inhibitors in soil DNA extraction.

Related tags

Soil Dna ExtractionHumic SubstancesPcr InhibitorsDna PurificationRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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