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When can humic acids be a problem in DNA isolation?

Answer and explanation

Correct answer: In soil or other environmental samples

Humic acids can co-extract with DNA from soil and many environmental samples. They may inhibit enzyme-based assays such as PCR, so an additional cleanup step can be needed during DNA purification. In a pure plasmid preparation, humic acids are not usually the main contaminant. Exam tip: remember humic acids as common PCR inhibitors in soil DNA extraction.

Related tags

Humic AcidsSoil DnaEnvironmental SamplesDna IsolationPcr InhibitorsDna PurificationIsolation Of DnaProcesses Of Recombinant Dna Technology

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