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Why is inhibitor removal necessary in DNA isolation?

Answer and explanation

Correct answer: To enable successful downstream PCR and enzyme reactions

Proteins, phenol, salts, and other contaminants in a DNA sample can inhibit enzyme-based reactions such as PCR and restriction digestion. Removing these inhibitors produces purer DNA and makes downstream tests more reliable. Inhibitor removal does not convert DNA into protein. Exam tip: DNA purity is essential before downstream steps such as PCR, sequencing, or restriction digestion.

Related tags

Dna IsolationInhibitor RemovalPcrRestriction EnzymesDna PurificationRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna Technology

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