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What is the best prevention against sample contamination in DNA extraction?

Answer and explanation

Correct answer: Using aseptic handling and fresh tips

The direct answer is option D: use aseptic handling and fresh tips. Contamination means unwanted DNA, microbes, chemicals or material from another sample entering the experiment. Prevention is better than trying to remove contamination later. Step by step: clean the work area; label tubes before starting; use sterile or clean disposable tips; change the tip whenever moving to another reagent or sample; keep tubes closed except when adding something; and avoid touching the tip to surfaces. Option A is wrong because leaving tubes open allows dust, droplets and microbes to enter. Option B is wrong because reusing a tip can carry material from one tube into another and cause cross-contamination. Option C is wrong because removing labels creates identification errors and can mix up samples; it does not prevent contamination. Option D is correct because aseptic technique and fresh tips reduce both environmental contamination and transfer between samples, giving more trustworthy DNA results. Remember: closed tubes, clean workspace, fresh tip, correct label. These simple habits protect the whole experiment.

Related tags

Aseptic HandlingFresh TipsContamination

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