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What is one benefit of keeping samples on ice in DNA isolation?

Answer and explanation

Correct answer: Nuclease enzyme activity may slow down

Keeping a sample on ice lowers the temperature and can slow the activity of nucleases. Since nucleases may break down DNA, cold conditions help reduce DNA degradation during isolation. DNA precipitation mainly depends on alcohol and salts, not simply on keeping the sample on ice. Exam tip: In DNA isolation, low temperature is used chiefly to protect DNA from enzyme-mediated degradation.

Related tags

Dna IsolationNuclease ActivityDna DegradationLow TemperatureRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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