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If DNA concentration is too high what should be done for PCR?

Answer and explanation

Correct answer: Dilute the DNA sample to an appropriate concentration

The amount of DNA template in PCR should be within an appropriate range. When DNA concentration is too high, the sample is diluted so that a suitable amount of template is added; excess DNA or associated impurities can interfere with PCR. Ethanol is used for DNA precipitation, not as the usual step to reduce an overly concentrated PCR template. Exam tip: If the template is too concentrated for PCR, dilution is generally the first step.

Related tags

PcrDna ConcentrationTemplate DnaDna DilutionRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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