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What can cause DNA shearing?

Answer and explanation

Correct answer: Harsh vortexing and rough pipetting

Mechanical forces produced by harsh vortexing and rough pipetting can break long genomic DNA molecules into smaller fragments; this is called DNA shearing. A correct buffer and proper cooling help maintain DNA stability and are not usual causes of shearing. Exam tip: To protect high-molecular-weight DNA during isolation, mix gently and use wide-bore pipette tips.

Related tags

Dna IsolationDna ShearingGenomic DnaVortexingPipettingRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna Technology

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