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Why do polysaccharides create difficulty in plant DNA samples?

Answer and explanation

Correct answer: They can co-purify with DNA and make the extract viscous

Polysaccharides from plant tissues may co-extract or co-purify with DNA. They make the DNA extract viscous and can inhibit enzymatic reactions such as PCR and restriction digestion. Option D is incorrect because polysaccharides do not synthesize DNA or increase its copy number. Exam tip: In plant DNA isolation, associate polysaccharide contamination with viscosity and enzyme inhibition.

Related tags

Plant Dna IsolationPolysaccharidesDna ExtractionViscosityPcr InhibitionCtab MethodIsolation Of DnaProcesses Of Recombinant Dna Technology

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