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What problem can interface contamination cause in a DNA sample?

Answer and explanation

Correct answer: Contamination with proteins and cellular debris

During DNA isolation, the interface between the aqueous and organic layers often contains proteins, cell debris, and other impurities. If this layer is accidentally collected, the DNA sample becomes contaminated with proteins and cellular debris, lowering its purity. Option A is the opposite effect. Exam tip: Carefully remove the DNA-containing aqueous layer without disturbing the interface.

Related tags

Dna IsolationInterface ContaminationDna PurityProtein ContaminationCell DebrisIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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