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What is the target DNA in plasmid DNA isolation?

Answer and explanation

Correct answer: small circular extra-chromosomal DNA / Small circular extra-chromosomal DNA

In a plasmid-DNA isolation procedure, the target is the plasmid molecule rather than the cell’s main chromosome. A plasmid is usually a small, circular, double-stranded DNA molecule that exists separately from the bacterial chromosome. It can replicate inside a suitable host cell and may carry useful genes, such as a selectable marker or an inserted recombinant sequence.

Thus, option B is correct: the aim of a plasmid preparation is to collect this small circular extra-chromosomal DNA. Chromosomal DNA may be unwanted carryover, while RNA and proteins are different biological materials and are removed during purification. The word “target” means the nucleic-acid product the procedure is designed to recover, not every substance present in the cell.

Related tags

Plasmid DnaCircular DnaExtra Chromosomal

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