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In genomic DNA isolation what may need to be kept more intact?

Answer and explanation

Correct answer: Long chromosomal DNA

Genomic DNA consists of long chromosomal DNA molecules. Vigorous vortexing, repeated pipetting, or rough mixing can shear these molecules into smaller fragments, so they should be kept as intact as possible. Breaking small RNA fragments is not the main concern during genomic DNA isolation. Exam tip: gentle mixing and wide-bore pipette tips help reduce DNA shearing.

Related tags

Genomic DnaIsolation Of DnaChromosomal DnaDna ShearingGentle HandlingRecombinant Dna TechnologyProcesses Of Recombinant Dna TechnologyBiotechnology

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