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Why is cold alcohol preferred in DNA precipitation?

Answer and explanation

Correct answer: To further reduce the solubility of DNA

Cold alcohol further decreases the solubility of DNA, allowing DNA to come out of solution and be recovered more effectively as a precipitate. Low temperature also reduces DNase activity, helping to limit DNA degradation. Converting DNA into protein is incorrect because DNA is a nucleic acid, not a protein. Exam tip: In DNA isolation, cold ethanol or isopropanol is added after salt treatment to precipitate DNA.

Related tags

Dna IsolationDna PrecipitationCold AlcoholDna SolubilityRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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