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Why is salt added in DNA precipitation?

Answer and explanation

Correct answer: To neutralise the negative phosphate charges of DNA

The phosphate groups in DNA carry negative charges. Cations from the salt neutralise or shield these charges, reducing repulsion between DNA molecules and allowing them to aggregate and precipitate in the presence of alcohol. Alcohol lowers DNA solubility, but it does not neutralise the phosphate charges. Exam tip: remember that salt mainly neutralises the negative charges on the DNA phosphate backbone.

Related tags

Dna IsolationDna PrecipitationSaltPhosphate BackboneRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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