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How does EDTA reduce nuclease activity in DNA isolation?

Answer and explanation

Correct answer: By chelating metal ions

Many DNases and other nucleases require divalent metal ions such as \(Mg^{2+}\) for their activity. EDTA chelates these ions, making them unavailable to the enzyme; this reduces nuclease activity and protects the DNA. Cutting proteins is the role of proteases, not EDTA. Exam tip: Remember EDTA as a metal-ion chelator used to protect DNA during isolation.

Related tags

EdtaDna IsolationNuclease InhibitionMetal Ion ChelationRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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