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Why should repeatedly drawing a DNA sample through a pipette tip be avoided?

Answer and explanation

Correct answer: To reduce the risk of shearing long DNA molecules

Repeated pipetting forces long DNA molecules through the narrow pipette tip, creating mechanical stress that can shear them into smaller fragments. DNA samples are therefore mixed gently to preserve DNA integrity. Pipetting does not increase DNA concentration or remove RNA contamination. Exam tip: Handle high-molecular-weight genomic DNA gently; wide-bore tips can help reduce shearing.

Related tags

Dna IsolationDna ShearingPipettingGenomic DnaDna IntegrityRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna Technology

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