If DNA purity is poor but yield is high, what should be done?यदि DNA की purity खराब हो लेकिन yield अधिक हो, तो क्या करना चाहिए?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Consider additional cleanupConsider additional cleanup
A high DNA yield does not guarantee that the preparation is suitable for downstream work. Poor purity may indicate carryover of proteins, phenol, salts, carbohydrates, RNA, or other extraction reagents. Such contaminants can inhibit restriction digestion, PCR, ligation, sequencing, or accurate concentration measurement. The sample should first be assessed using appropriate quality checks and then subjected to an additional cleanup, re-purification, or other validated treatment selected for the contaminant. Option A is correct because it aims to retain the valuable DNA while removing inhibitory substances. Using it without checking risks unreliable results; automatic disposal wastes potentially recoverable material, and adding protein would worsen contamination.DNA की yield अधिक होना यह सिद्ध नहीं करता कि preparation downstream प्रयोगों के लिए उपयुक्त है। खराब purity का कारण proteins, phenol, salts, carbohydrates, RNA या extraction reagents का carryover हो सकता है। ये contaminants restriction digestion, PCR, ligation, sequencing और concentration measurement को बाधित कर सकते हैं। इसलिए पहले उचित quality check करना चाहिए और फिर contaminant के अनुसार additional cleanup, re-purification या कोई validated treatment अपनाना चाहिए। विकल्प A सही है, क्योंकि इससे उपयोगी DNA बचाते हुए inhibitory substances हटाए जा सकते हैं। बिना जाँच sample इस्तेमाल करने से परिणाम अविश्वसनीय हो सकते हैं; हर बार DNA फेंकना अनावश्यक है और protein मिलाने से contamination बढ़ेगा।
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