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Why can duplicate extraction be done in DNA isolation?

Answer and explanation

Correct answer: To check result reliability

The direct answer is A: duplicate extraction is done to check result reliability. A duplicate means carrying out the extraction independently two times, preferably from the same relevant sample or from matched material. The reasoning is: perform extraction 1; perform extraction 2; measure DNA yield or quality in both; compare the results; similar results support reproducibility, while a large difference signals possible pipetting error, sample inconsistency, contamination, inhibition, or a procedural problem that needs investigation. Option A is correct because repeated independent results increase confidence that the finding is genuine rather than accidental. Option B is wrong because duplication is not intended to destroy DNA. Option C is irrelevant and has no scientific role in DNA extraction. Option D is wrong because extraction does not aim to make a cell wall; cell walls are biological structures made by cells. Duplicate work does not guarantee perfection, but it is a useful quality-control check. Memory cue: duplicate means “do it again and compare.”

Related tags

Duplicate ExtractionReliabilityReproducibility

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