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What effect can salt carryover have in a DNA sample?

Answer and explanation

Correct answer: Downstream enzyme reactions may be inhibited

Salt left over after DNA isolation can reduce the efficiency of PCR, restriction-enzyme digestion, and other enzyme-based reactions. Excess salt interferes with enzyme activity and the required ionic conditions of the reaction. It does not permanently make DNA single-stranded or spontaneously alter its sequence. Exam tip: the wash step during DNA purification helps remove inhibitors such as salts.

Related tags

Dna IsolationSalt CarryoverEnzyme InhibitionPcrRestriction DigestionDna PurificationIsolation Of DnaProcesses Of Recombinant Dna Technology

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