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What can sodium acetate do in DNA precipitation?

Answer and explanation

Correct answer: Provide sodium ions to neutralise the negative phosphate charges on DNA

Na⁺ ions supplied by sodium acetate shield or neutralise the negative charges of DNA phosphate groups. This reduces repulsion between DNA molecules, allowing DNA to precipitate when ethanol or isopropanol is added. Breaking the sugar-phosphate backbone would damage DNA, so it is not the role of sodium acetate. Exam tip: remember that salt reduces the effective negative charge on DNA during precipitation.

Related tags

Dna PrecipitationSodium AcetateDna IsolationRecombinant Dna TechnologyNucleic Acid ExtractionIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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