Update

Muft Shiksha™ एक 100% Free Education Portal है 🇮🇳, जिसका उद्देश्य Class 9–12 के हर विद्यार्थी तक High-Quality Education को पूरी तरह मुफ्त पहुँचाना है। 🇮🇳 हम मानते हैं कि अच्छी शिक्षा किसी student की आर्थिक स्थिति पर निर्भर नहीं होनी चाहिए। 🇮🇳 हर विद्यार्थी को वही Quality Study Material, MCQs, Quizzes, Exam Preparation, Concept-Based Learning और Bilingual Support मिलना चाहिए, जो आमतौर पर महंगी Coaching या Premium Platforms में मिलता है। Muft Shiksha™ 🇮🇳 इसी सोच के साथ बनाया गया है

Subjects
0 reads0 ratings0 helpful

Why is caution needed while taking the aqueous phase in DNA isolation?

Answer and explanation

Correct answer: To avoid contamination by proteins and other impurities from the interface

After centrifugation during DNA isolation, DNA is usually present in the aqueous phase, while the interface may contain proteins, cellular debris, and other impurities. Carefully removing the aqueous phase prevents the interface from being carried over and keeps the DNA purer. Protein synthesis and cell growth are not purposes of this step. Exam tip: keep the pipette tip away from the interface while collecting the aqueous layer.

Related tags

Dna IsolationAqueous PhaseInterface ContaminationDna PurityOrganic ExtractionIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

Was this question useful?

No ratings yetWrite a review / Rate this question

Student Reviews

No published reviews yet.

Add Muft Shiksha to your Home Screen

In Safari, tap Share, then Add to Home Screen.